제품 > 항체 > 다중클론항체(pAb)

RIP3 Rabbit pAb (A25050)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunohistochemistry analysis of RIP3 in paraffin-embedded rat kidney tissue using RIP3 Rabbit pAb (A25050) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of RIP3 in paraffin-embedded mouse intestin tissue using RIP3 Rabbit pAb (A25050) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of RIP3 in paraffin-embedded human colon carcinoma tissue using RIP3 Rabbit pAb (A25050) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of RIP3 in paraffin-embedded human tonsil tissue using RIP3 Rabbit pAb (A25050) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RIP3 Rabbit pAb (A25050)

Immunofluorescence analysis of L-929 cells using RIP3 Rabbit pAb(A25050) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	RIP3 Rabbit pAb
Catalog No.	A25050
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables NF-kappaB-inducing kinase activity and protein serine/threonine kinase activity. Involved in several processes, including execution phase of necroptosis; hematopoietic or lymphoid organ development; and regulation of programmed cell death. Acts upstream of or within several processes, including I-kappaB kinase/NF-kappaB signaling; positive regulation of intrinsic apoptotic signaling pathway; and positive regulation of protein deacetylation. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; hemolymphoid system gland; male reproductive gland or organ; primitive streak; and turbinate bone primordium. Orthologous to human RIPK3 (receptor interacting serine/threonine kinase 3).

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 320-420 of mouse RIP3(NP_064339.2).
Sequence	LSAREPSQRGTEMDCPRETMVSKMLDRLHLEEPSGPVPGKCPERQAQDTSVGPATPARTSSDPVAGTPQIPHTLPFRGTTPGPVFTETPGPHPQRNQGDGR
Gene ID
Swiss Prot
Synonyms	Rip3; 2610528K09Rik; RIP3
Calculated MW	53kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Immunohistochemistry Immunofluorescence
Positive samples
Cellular location	Cell membrane, Cytoplasm, Mitochondrion, cytosol