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RPA70/RPA1 Rabbit pAb (A0874)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - RPA70/RPA1 Rabbit pAb (A0874)

Western blot analysis of various lysates using RPA70/RPA1 Rabbit pAb (A0874) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - RPA70/RPA1 Rabbit pAb (A0874)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using RPA70/RPA1 Rabbit pAb (A0874) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPA70/RPA1 Rabbit pAb (A0874)

Immunohistochemistry analysis of paraffin-embedded human uterus using RPA70/RPA1 Rabbit pAb (A0874) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameRPA70/RPA1 Rabbit pAb
Catalog No.A0874
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RPA70/RPA70/RPA1 (NP_002936.1).
SequenceMVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELEVLKSAEAVGVKIGNPVPYNEGLGQPQVAPPAPAASPAASSRPQPQNGSSGMGSTVSKAYGASKTFGKAAGPSLSHTSGGTQSKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGEGKLFSLELVDESGEIRATAFNE
Gene ID
Swiss Prot
SynonymsHSSB; RF-A; RP-A; REPA1; RPA70; MST075; PFBMFT6; RPA70/RPA1
Calculated MW68kDa
Observed MW70kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, HeLa, 293T, A-549
Cellular locationNucleus, PML body

Documents

Certificate of Compliance

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ABclonal:Western blot - RPA70/RPA1 Rabbit pAb (A0874)}

Western blot - RPA70/RPA1 Rabbit pAb (A0874)

Western blot analysis of various lysates using RPA70/RPA1 Rabbit pAb (A0874) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - RPA70/RPA1 Rabbit pAb (A0874)}

Immunohistochemistry - RPA70/RPA1 Rabbit pAb (A0874)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using RPA70/RPA1 Rabbit pAb (A0874) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPA70/RPA1 Rabbit pAb (A0874)}

Immunohistochemistry - RPA70/RPA1 Rabbit pAb (A0874)

Immunohistochemistry analysis of paraffin-embedded human uterus using RPA70/RPA1 Rabbit pAb (A0874) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)