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RPL23A Rabbit pAb (A4086)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPL23A Rabbit pAb (A4086)

Western blot analysis of various lysates using RPL23A Rabbit pAb (A4086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - RPL23A Rabbit pAb (A4086)

Immunohistochemistry analysis of paraffin-embedded rat brain using RPL23A Rabbit pAb (A4086) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL23A Rabbit pAb (A4086)

Immunohistochemistry analysis of paraffin-embedded human esophageal using RPL23A Rabbit pAb (A4086) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL23A Rabbit pAb (A4086)

Immunohistochemistry analysis of paraffin-embedded human placenta using RPL23A Rabbit pAb (A4086) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPL23A Rabbit pAb (A4086)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using RPL23A Rabbit pAb (A4086) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - RPL23A Rabbit pAb (A4086)

Immunofluorescence analysis of A-431 cells using RPL23A Rabbit pAb (A4086) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL23A Rabbit pAb (A4086)

Immunofluorescence analysis of HeLa cells using RPL23A Rabbit pAb (A4086) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RPL23A Rabbit pAb (A4086)

Confocal immunofluorescence analysis of HeLa cells using RPL23A Rabbit pAb (A4086) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameRPL23A Rabbit pAb
Catalog No.A4086
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins. It is located in the cytoplasm. The protein may be one of the target molecules involved in mediating growth inhibition by interferon. In yeast, the corresponding protein binds to a specific site on the 26S rRNA. This gene is co-transcribed with the U42A, U42B, U101A, and U101B small nucleolar RNA genes, which are located in its third, first, second, and fourth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human RPL23A (NP_000975.2).
SequenceMAPKAKKEAPAPPKAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII
Gene ID
Swiss Prot
SynonymsL23A; uL23; MDA20; RPL23A
Calculated MW18kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, HT-1080, COS-7, HeLa, Jurkat, MCF7, Mouse lung, Mouse brain, Rat brain
Cellular locationcytoplasm, cytosol, cytosolic ribosome, extracellular exosome, nucleolus, nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - RPL23A Rabbit pAb (A4086)}

Western blot - RPL23A Rabbit pAb (A4086)

Western blot analysis of various lysates using RPL23A Rabbit pAb (A4086) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - RPL23A Rabbit pAb (A4086)}

Immunohistochemistry - RPL23A Rabbit pAb (A4086)

Immunohistochemistry analysis of paraffin-embedded rat brain using RPL23A Rabbit pAb (A4086) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL23A Rabbit pAb (A4086)}

Immunohistochemistry - RPL23A Rabbit pAb (A4086)

Immunohistochemistry analysis of paraffin-embedded human esophageal using RPL23A Rabbit pAb (A4086) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL23A Rabbit pAb (A4086)}

Immunohistochemistry - RPL23A Rabbit pAb (A4086)

Immunohistochemistry analysis of paraffin-embedded human placenta using RPL23A Rabbit pAb (A4086) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPL23A Rabbit pAb (A4086)}

Immunohistochemistry - RPL23A Rabbit pAb (A4086)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using RPL23A Rabbit pAb (A4086) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - RPL23A Rabbit pAb (A4086)}

Immunofluorescence - RPL23A Rabbit pAb (A4086)

Immunofluorescence analysis of A-431 cells using RPL23A Rabbit pAb (A4086) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPL23A Rabbit pAb (A4086)}

Immunofluorescence - RPL23A Rabbit pAb (A4086)

Immunofluorescence analysis of HeLa cells using RPL23A Rabbit pAb (A4086) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RPL23A Rabbit pAb (A4086)}

Immunofluorescence - RPL23A Rabbit pAb (A4086)

Confocal immunofluorescence analysis of HeLa cells using RPL23A Rabbit pAb (A4086) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)