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RPS2 Rabbit pAb (A6728)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS2 Rabbit pAb (A6728)

Western blot analysis of various lysates using RPS2 Rabbit pAb (A6728) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunohistochemistry - RPS2 Rabbit pAb (A6728)

Immunohistochemistry analysis of paraffin-embedded Human stomach using RPS2 Rabbit pAb (A6728) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPS2 Rabbit pAb (A6728)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using RPS2 Rabbit pAb (A6728) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - RPS2 Rabbit pAb (A6728)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using RPS2 Rabbit pAb (A6728) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameRPS2 Rabbit pAb
Catalog No.A6728
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S5P family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with mouse LLRep3. It is co-transcribed with the small nucleolar RNA gene U64, which is located in its third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human RPS2 (NP_002943.2).
SequenceMADDAGAAGGPGGPGGPGMGNRGGFRGGFGSGIRGRGRGRGRGRGRGRGARGGKAEDKEWMPVTKLGRLVKDMKIKSLEEIYLFSLPIKESEIIDFFLGASLKDEVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKLSIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPAPRGTGIVSAPVPKKLLMMAGIDDCYTSARGCTATLGNFAKATFDAISKTYSYLTPDLWKETVFTKSPYQEFTDHLVKTHTRVSVQRTQAPAVATT
Gene ID
Swiss Prot
SynonymsS2; uS5; LLREP3; RPS2
Calculated MW31kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, U-251MG, MCF7, HeLa
Cellular locationcytoplasm, cytosol, cytosolic ribosome, extracellular exosome, focal adhesion, nucleoplasm, nucleus
Customer validation

WB(Homo sapiens, Eriocheir sinensis H.Milne-Edwards, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - RPS2 Rabbit pAb (A6728)}

Western blot - RPS2 Rabbit pAb (A6728)

Western blot analysis of various lysates using RPS2 Rabbit pAb (A6728) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunohistochemistry - RPS2 Rabbit pAb (A6728)}

Immunohistochemistry - RPS2 Rabbit pAb (A6728)

Immunohistochemistry analysis of paraffin-embedded Human stomach using RPS2 Rabbit pAb (A6728) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPS2 Rabbit pAb (A6728)}

Immunohistochemistry - RPS2 Rabbit pAb (A6728)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using RPS2 Rabbit pAb (A6728) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - RPS2 Rabbit pAb (A6728)}

Immunohistochemistry - RPS2 Rabbit pAb (A6728)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using RPS2 Rabbit pAb (A6728) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6728? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)