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RPS3 Rabbit mAb (A4872)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RPS3 Rabbit mAb (A4872)

Western blot analysis of extracts of various cell lines, using RPS3 Rabbit mAb (A4872) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - RPS3 Rabbit mAb (A4872)

Immunofluorescence analysis of NIH-3T3 cells using RPS3 Rabbit mAb (A4872) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameRPS3 Rabbit mAb
Catalog No.A4872
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0302
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human RPS3 (P23396).
SequenceAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGP
Gene ID
Swiss Prot
SynonymsS3; uS3
Calculated MW27kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, HT-29, Mouse liver, Mouse spleen, Mouse pancreas, Rat lung
Cellular locationCytoplasm, Mitochondrion inner membrane, Nucleus, Peripheral membrane protein, cytoskeleton, nucleolus, spindle
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - RPS3 Rabbit mAb (A4872)}

Western blot - RPS3 Rabbit mAb (A4872)

Western blot analysis of extracts of various cell lines, using RPS3 Rabbit mAb (A4872) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - RPS3 Rabbit mAb (A4872)}

Immunofluorescence - RPS3 Rabbit mAb (A4872)

Immunofluorescence analysis of NIH-3T3 cells using RPS3 Rabbit mAb (A4872) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4872? Please let us know so that we can cite the reference in this datasheet.

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