Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using RSK2 / RPS6KA3 Rabbit mAb (A20868) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of lysates from NIH/3T3 cells, using RSK2 / RPS6KA3 Rabbit mAb (A20868) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of RSK2 / RPS6KA3 in paraffin-embedded human breast cancer tissue using RSK2 / RPS6KA3 Rabbit mAb (A20868) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of RSK2 / RPS6KA3 in paraffin-embedded human liver tissue using RSK2 / RPS6KA3 Rabbit mAb (A20868) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of RSK2 / RPS6KA3 in paraffin-embedded rat intestine tissue using RSK2 / RPS6KA3 Rabbit mAb (A20868) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of RSK2 / RPS6KA3 in paraffin-embedded mouse lung tissue using RSK2 / RPS6KA3 Rabbit mAb (A20868) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of RSK2 / RPS6KA3 in paraffin-embedded mouse intestin tissue using RSK2 / RPS6KA3 Rabbit mAb (A20868) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
| Product name | RSK2 / RPS6KA3 Rabbit mAb |
|---|---|
| Catalog No. | A20868 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC2799 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 600-700 of human RSK2 / RPS6KA3 (P51812). |
|---|---|
| Sequence | DIWSLGVLLYTMLTGYTPFANGPDDTPEEILARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; XLID19; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3; RSK2 / RPS6KA3 |
| Calculated MW | 84kDa |
| Observed MW | 84kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | HeLa, HepG2, NIH/3T3 |
| Cellular location | Cytoplasm, Nucleus |
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