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RYR2 Rabbit pAb (A0298)

Publications (7) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RYR2 Rabbit pAb (A0298)

Western blot analysis of lysates from Mouse heart, using RYR2 Rabbit pAb (A0298) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - RYR2 Rabbit pAb (A0298)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using RYR2 Rabbit pAb (A0298) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RYR2 Rabbit pAb (A0298)

Immunohistochemistry analysis of paraffin-embedded Human heart tissue using RYR2 Rabbit pAb (A0298) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RYR2 Rabbit pAb (A0298)

Immunofluorescence analysis of paraffin-embedded mouse heart using RYR2 Rabbit pAb (A0298) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRYR2 Rabbit pAb
Catalog No.A0298
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 4868-4967 of human RYR2 (NP_001026.2).
SequenceDAFGELRDQQEQVKEDMETKCFICGIGNDYFDTVPHGFETHTLQEHNLANYLFFLMYLINKDETEHTGQESYVWKMYQERCWEFFPAGDCFRKQYEDQLN
Gene ID
Swiss Prot
SynonymsRyR; ARVC2; ARVD2; RYR-2; VTSIP; VACRDS; RYR2
Calculated MW565kDa
Observed MW564kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse heart
Cellular locationMembrane, Multi-pass membrane protein, Sarcoplasmic reticulum membrane.
Customer validation

WB(Mus musculus,Rattus norvegicus, Gallus gallus)

IF(Mus musculus)

Documents

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ABclonal:Western blot - RYR2 Rabbit pAb (A0298)}

Western blot - RYR2 Rabbit pAb (A0298)

Western blot analysis of lysates from Mouse heart, using RYR2 Rabbit pAb (A0298) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - RYR2 Rabbit pAb (A0298)}

Immunohistochemistry - RYR2 Rabbit pAb (A0298)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using RYR2 Rabbit pAb (A0298) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - RYR2 Rabbit pAb (A0298)}

Immunohistochemistry - RYR2 Rabbit pAb (A0298)

Immunohistochemistry analysis of paraffin-embedded Human heart tissue using RYR2 Rabbit pAb (A0298) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - RYR2 Rabbit pAb (A0298)}

Immunofluorescence - RYR2 Rabbit pAb (A0298)

Immunofluorescence analysis of paraffin-embedded mouse heart using RYR2 Rabbit pAb (A0298) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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