| Product name | Rabbit anti-Chicken IL-10 mAb (CAP) |
|---|---|
| Catalog No. | RM17835 |
| Catagory | Elisa Antibody Kit |
| Application | ELISA |
| Description | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation |
| Immunogen | Recombinant |
| Purirty | Affinity purification |
| Ig Type | Rabbit IgG |
| Formulation | Supplied as a 0.2um filtered solution in PBS with 0.05%ProClin 300, PH 7.4. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Preservative：0.05%ProClin 300. Avoid repeated freeze-thaw cycles. |
Chicken IL-10 Sandwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 0.5-4ug/mL | Rabbit anti-Chicken IL-10 (CAP)(Cat. No.RM17835) |
| ELISA Detection | 0.1-0.4ug/mL | Rabbit anti-Chicken IL-10 (DET)(Cat. No.RM17836) |
| Standard | 62.5-4000pg/mL | Recombinant Chicken IL-10 Protein |
