| Product name | Rabbit anti-Human IL-4 mAb (DET) |
|---|---|
| Catalog No. | RM17745 |
| Catagory | Elisa Antibody Kit |
| Application | CLIA |
| Description | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Immunogen | Recombinant Human IL-4 Protein |
| Purirty | Affinity purification |
| Ig Type | Rabbit IgG |
| Formulation | Supplied as a 0.2umfiltered solution in PBS with0.1%Braveds MB-1 , Ph7.4. |
| CrossReactivity | No cross-reactivity in ELISA assay with recombinant hIL-13, mGM-CSF , mIL-13, mIL-4, rIL-4, rIL-4 |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Preservative：0.05%ProClin 300. Avoid repeated freeze-thaw cycles. |
Human IL-4 Sanwich CLIA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| CLIA Capture | 1-4ug/mL | Rabbit anti-Human IL-4(CAP)(Cat. No.RM17744) |
| CLIA Detection | 0.5-2ug/mL | Rabbit anti-Human IL-4(DET)(Cat. No.RM17745) |
| Standard | 0.1-10ng/mL | Recombinant Human IL-4 Protein |
