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Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)

Datasheet

ABclonal: - Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)
ABclonal: - Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)
ABclonal: - Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)

Overview

Product nameRabbit anti-Mouse TNF-α mAb (CAP)
Catalog No.RM17776
CatagoryElisa Antibody Kit
ApplicationELISA
DescriptionThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant Mouse TNF-α Protein
PurirtyAffinity purification
Ig TypeRabbit IgG
FormulationSupplied as a 0.2umfiltered solution in PBS with0.05%ProClin 300 , Ph7.4 containing Mouse TNF-α Antibody.
CrossReactivityHas cross-reactivity in ELISA with
Rat TNF-α
No cross-reactivity in ELISA assay with recombinant
Mouse IL-1α、Mouse IL-1β、Mouse IL-2、Mouse IL-4、Mouse IL-5、Mouse IL-6、Mouse IL-10、Mouse IL-12/IL-23 p40、Mouse IFN-β、Mouse IFN-γ、Mouse EGF、Human TNF-α
StorageStore at -20℃. Avoid freeze / thaw cycles. Preservative:0.05%ProClin 300.

Avoid repeated freeze-thaw cycles.

Application

Mouse TNF-α Sandwich ELISA Immunoassay

 Recommended
Concentration
Sample
ELISA Capture1-4ug/mLRabbit anti-Mouse TNF-α(CAP)(Cat. No.RM17776)
ELISA Detection0.04-0.16ug/mLRabbit anti-Mouse TNF-α(DET)(Cat. No.RM17777)
Standard2.3-150pg/mLRecombinant Mouse TNF-α Protein(Cat. No.RP01071)
ABclonal: - Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)}

- Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)

This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
ABclonal: - Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)}

- Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)

Fromthe comparison of the standard curve, it is found that ABclonal has highsensitivity.
ABclonal: - Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)}

- Rabbit anti-Mouse TNF-α mAb (CAP) (RM17776)

Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
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