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Raf1 Rabbit pAb (A0223)

Reviews (3)Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Raf1 Rabbit pAb (A0223)

Western blot analysis of lysates from Mouse liver, using Raf1 Rabbit pAb (A0223) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Raf1 Rabbit pAb (A0223)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Raf1 Rabbit pAb (A0223) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Raf1 Rabbit pAb (A0223)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Raf1 Rabbit pAb (A0223) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Raf1 Rabbit pAb (A0223)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using Raf1 Rabbit pAb (A0223) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Raf1 Rabbit pAb (A0223)

Immunofluorescence analysis of PC-12 cells using Raf1 Rabbit pAb (A0223) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Raf1 Rabbit pAb(A0223)
Review
ABclonal: review for Raf1 Rabbit pAb(A0223)
Review
ABclonal: review for Raf1 Rabbit pAb(A0223)

Overview

Product nameRaf1 Rabbit pAb
Catalog No.A0223
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human Raf1 (NP_002871.1).
SequenceIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVM
Gene ID
Swiss Prot
SynonymsNS5; CRAF; Raf-1; c-Raf; CMD1NN; Raf1
Calculated MW73kDa
Observed MW73kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver
Cellular locationCell membrane, Cytoplasm, Mitochondrion, Nucleus.
Customer validation

(Human lung, Murine cells, Mouse spleen)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Raf1 Rabbit pAb (A0223)}

Western blot - Raf1 Rabbit pAb (A0223)

Western blot analysis of lysates from Mouse liver, using Raf1 Rabbit pAb (A0223) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Raf1 Rabbit pAb (A0223)}

Immunohistochemistry - Raf1 Rabbit pAb (A0223)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Raf1 Rabbit pAb (A0223) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Raf1 Rabbit pAb (A0223)}

Immunohistochemistry - Raf1 Rabbit pAb (A0223)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Raf1 Rabbit pAb (A0223) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Raf1 Rabbit pAb (A0223)}

Immunohistochemistry - Raf1 Rabbit pAb (A0223)

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using Raf1 Rabbit pAb (A0223) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Raf1 Rabbit pAb (A0223)}

Immunofluorescence - Raf1 Rabbit pAb (A0223)

Immunofluorescence analysis of PC-12 cells using Raf1 Rabbit pAb (A0223) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

Secondary Antibodies (26)