The Rapid Plus DNA Lib Prep kit provides a streamlined and efficient method to construction DNA library for next generation sequencing (NGS) at Illumina® platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 - 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free library from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification. The kit also provides DNA repair enzymes, which could repair DNA damages such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase the NGS library yields from FFPE-derived DNA.
Workflow Chart
Storage
All components should be stored at -20°C. The shelf lives of all reagents are one year when stored properly.
Product Information
Product Components
Tube name
8 RXN
24 RXN
96 RXN
End Preparation
End Prep Buffer
70 μl
168 μl
700 μl
End Prep Enzymes
30 μl
78 μl
300 μl
Damaged DNA Repair Enzymes
30 μl
78 μl
300 μl
Adaptor Ligation
Ligation Buffer
300 μl
780 μl
3 ml
Ligase Enzymes
100 μl
240 μl
1000 μl
Amplification
2X PCR Master Mix
250 μl
650 μl
2.5 mL
10X PCR Primers
50 μl
130 μl
500 μl
Product Data
1.Library construction using FFPE DNA and Tissue DNA
Phase 1 multicenter, dose-expansion study of ARX788 as monotherapy in HER2-positive advanced gastric and gastroesophageal junction adenocarcinoma - PMC
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