Rapid Plus DNA Lib Prep Kit for Illumina V2继承了Rapid Plus DNA Lib Prep Kit的高性能，在基础上进一步提高了低投入量连接效率并提高了DNA损伤修复功能，是一款通用型的文库构建试剂盒。
本试剂盒可将1 ng~1000 ng的DNA样本构建成适用于Illumina®高通量测序平台的DNA测序文库。试剂盒包含了DNA样本末端修复、加dA、测序接头连接和文库扩增等一系列建库实验所需的酶及反应Buffer，便于实现文库的快速构建，整个实验过程可以在2～3 hr内完成，适用于各种样本类型（普通基因组、游离DNA、FFPE DNA、CHIP DNA等）, 另外本试剂盒还可以用于PCR-free文库构建（例如：高质量基因组Input DNA 100 ng）。ceshi
DNA from different species was used as template, and RK20255 was used for DNA library construction. Except for FFPE, the input amount of DNA was 100 ng, and the library was amplified by 8 cycles. The results showed that for different template types, the yield of the library constructed by RK20255 met the standard of on-machine sequencing.
2. Broad starting input
DNA library construction was performed using RK20255 with different starting amounts of Blood and Rice gDNA as templates. The results showed that the libraries constructed by RK20255 had high yield and could be compatible with a wider range of sample starting quantities.
3. Excellent library yield
DNA from different species was used as template, and RK20255 was used for DNA library construction. Except for FFPE, the input amount of DNA was 100 ng, and the library was amplified by 8 cycles. The results showed that the yield of the library constructed by RK20255 was higher than that of its competitors.
4. Excellent sequencing data quality
sample
product
Fragment size
The original Reads
Proportion of valid data
GC content
>Q20
>Q30
Mapping ratio
human blood gDNA
Rapid CE V2
194
1733332
99.36%
42.79%
97.56%
93.09%
97.38%
K*
201
1733332
99.29%
42.03%
97.42%
92.75%
97.54%
N*
186
1733332
99.29%
44.13%
97.43%
92.78%
96.44%
V*
196
1733332
99.10%
41.75%
97.40%
92.69%
97.73%
Y*
189
1733332
98.99%
42.41%
97.25%
92.37%
97.20%
Human Blood gDNA was used as template, and RK20255 and competing products were used for library construction and sequencing, respectively. Sequencing results showed that RK20255 had better sequencing quality consistency than its competitors.
5. Excellent accuracy
The base mutation rate of different FFPE DNA libraries was analyzed, and the results showed that the base mutation rate of RK20255 was lower than that of its competitors.
DNA library was constructed and sequenced by RK20255 using DNA from different species as template. The results showed that the proportion of chimeras in the library constructed by RK20254 was better than that of some competitors.
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