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Renin Rabbit mAb (A24393)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Renin Rabbit mAb (A24393)

Western blot analysis of various lysates, using Renin Rabbit mAb (A24393) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunofluorescence - Renin Rabbit mAb (A24393)

Confocal imaging of 293T-Renin and 293T cells using Renin Rabbit mAb (A24393, dilution 1:200)(Red). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product nameRenin Rabbit mAb
Catalog No.A24393
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63715
This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis.
ImmunogenRecombinant Protein corresponding to a sequence within amino acids 250-406AA of human Renin (NP_000528.1).
SequenceQHYEGNFHYINLIKTGVWQIQMKGVSVGSSTLLCEDGCLALVDTGASYISGSTSSIEKLMEALGAKKRLFDYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFQESYSSKKLCTLAIHAMDIPPPTGPTWALGATFIRKFYTEFDRRNNRIGFALAR
Gene ID
Swiss Prot
SynonymsRTD; HNFJ2; ADTKD4; Renin
Calculated MW45kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Mouse lung, Mouse ovary, Rat kidney, Rat lung
Cellular locationMembrane, Secreted.

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ABclonal:Western blot - Renin Rabbit mAb (A24393)}

Western blot - Renin Rabbit mAb (A24393)

Western blot analysis of various lysates, using Renin Rabbit mAb (A24393) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunofluorescence - Renin Rabbit mAb (A24393)}

Immunofluorescence - Renin Rabbit mAb (A24393)

Confocal imaging of 293T-Renin and 293T cells using Renin Rabbit mAb (A24393, dilution 1:200)(Red). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

ELISA 키트 (1)

Secondary Antibodies (26)