제품 > 항체 > 단일클론항체(mAb)

SARS-CoV-2 N Protein Mouse mAb (A20142)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:SARS-CoV-2

ABclonal:Western blot - SARS-CoV-2 N Protein Mouse mAb (A20142)

Western blot analysis of extracts of normal 293T cells 293T transfected with N Protein, using SARS-CoV-2 N Protein Mouse mAb (A20142) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal: - SARS-CoV-2 N Protein Mouse mAb (A20142)

Immobilized Recombinant 2019-nCoV Nucleocapsid Protein (RP01264LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Rabbit mAb (A20142) with a linear range of 3.12-200ng/mL.

ABclonal:Immunofluorescence - SARS-CoV-2 N Protein Mouse mAb (A20142)

Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 N Protein Mouse mAb (A20142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	SARS-CoV-2 N Protein Mouse mAb
Catalog No.	A20142
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG1, kappa
CloneNo.	AMC0368

Background

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9).
Sequence	MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADST
Gene ID
Swiss Prot
Synonyms	Nucleoprotein; NP; SARS-CoV-2 N Protein
Calculated MW	46kDa
Observed MW	50kDa

Applications

Reactivity	SARS-CoV-2
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IF/ICC 1:50 - 1:200 ELISA 1:30000 - 1:120000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T
Cellular location
Customer validation	ELISA(Mustela putorius furo) WB(Homo sapiens, Mus musculus) IP(Mus musculus) IF(Homo sapiens) IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

SARS-CoV-2 N Protein Mouse mAb images

Western blot - SARS-CoV-2 N Protein Mouse mAb (A20142)

- SARS-CoV-2 N Protein Mouse mAb (A20142)

Immobilized Recombinant 2019-nCoV Nucleocapsid Protein (RP01264LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Rabbit mAb (A20142) with a linear range of 3.12-200ng/mL.

Immunofluorescence - SARS-CoV-2 N Protein Mouse mAb (A20142)

Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 N Protein Mouse mAb (A20142) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.