Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:SARS-CoV-2
Western blot analysis of Recombinant SARS-CoV-2 Nucleocapsid Protein (RP01264LQ), using SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free (A20021) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 ng/10 ng/5 ng/2 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
| Product name | SARS-CoV-2 N Protein Rabbit mAb, BSA and glycerol free |
|---|---|
| Catalog No. | A20021 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC5077-02 |
| Immunogen | Recombinant fusion protein of SARS-CoV-2 Nucleoprotein Full length（P59595）. |
|---|---|
| Sequence | MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA |
| Gene ID | |
| Swiss Prot | |
| Synonyms | Nucleoprotein; NP; SARS-CoV-2 N Protein |
| Calculated MW | 46kDa |
| Observed MW |
| Reactivity | SARS-CoV-2 |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃ Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | Recombinant SARS-COV-2 N Protein |
| Cellular location | |
| Customer validation | WB(SARS-CoV-2, Staphylococcus aureus, Homo sapiens, Mus musculus) IHC(Homo sapiens, SARS-CoV-2, Rattus norvegicus) IF(Homo sapiens) IF(Homo sapiens) ELISA(Mus musculus) WB(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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