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SARS-CoV-2 NSP1 Rabbit pAb (A20200)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:SARS-CoV-2

ABclonal:SARS-CoV-2 NSP1 Rabbit pAb
ABclonal:Western blot - SARS-CoV-2 NSP1 Rabbit pAb (A20200)

Western blot analysis of extracts of normal 293T cells 293T transfected with NSP1 Protein, using SARS-CoV-2 NSP1 Rabbit pAb (A20200) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunoprecipitation - SARS-CoV-2 NSP1 Rabbit pAb (A20200)

Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg SARS-CoV-2 NSP1 antibody (A20200). Western blot was performed from the immunoprecipitate using SARS-CoV-2 NSP1 antibody (A20200) at a dilution of 1:1000.

Overview

Product nameSARS-CoV-2 NSP1 Rabbit pAb
Catalog No.A20200
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of coronavirus NSP1 (YP_009742608.1).
SequenceMESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG
Gene ID
Swiss Prot
Synonyms
Calculated MW141kDa
Observed MW20kDa
ReactivitySARS-CoV-2
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samples293T
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - SARS-CoV-2 NSP1 Rabbit pAb (A20200)}

    Western blot - SARS-CoV-2 NSP1 Rabbit pAb (A20200)

    Western blot analysis of extracts of normal 293T cells 293T transfected with NSP1 Protein, using SARS-CoV-2 NSP1 Rabbit pAb (A20200) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunoprecipitation - SARS-CoV-2 NSP1 Rabbit pAb (A20200)}

    Immunoprecipitation - SARS-CoV-2 NSP1 Rabbit pAb (A20200)

    Immunoprecipitation analysis of 600 μg extracts of 293T cells using 3 μg SARS-CoV-2 NSP1 antibody (A20200). Western blot was performed from the immunoprecipitate using SARS-CoV-2 NSP1 antibody (A20200) at a dilution of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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