제품 > 항체 > 다중클론항체(pAb)

SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:SARS-CoV-2

ABclonal:Western blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of Recombinant SARS-CoV-2 Nucleocapsid Protein (RP01264LQ)，using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 100ng/50ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Elisa - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immobilized Recombinant SARS-COV-2 Nucleocapsid (RP01264) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Antibody (A18797) with a linear range of 3.12-200ng/mL.

ABclonal:Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SARS-CoV-2 Nucleoprotein antibody (A18797). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (A18797) at a dilution of 1:1000.

Overview

Product name	SARS-CoV-2 Nucleoprotein Rabbit pAb
Catalog No.	A18797
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of coronavirus Nucleoprotein (P0DTC9).
Sequence	MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW	50kDa

Applications

Reactivity	SARS-CoV-2
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 DB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T transfected with Nucleoprotein, Recombinant SARS-CoV-2 Nucleoprotein
Cellular location
Customer validation	WB(Homo sapiens) Neutralization(Other)

Documents

Certificate of Compliance

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Lot number

Research Area

SARS-CoV-2 Nucleoprotein Rabbit pAb images

Western blot - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Elisa - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immobilized Recombinant SARS-COV-2 Nucleocapsid (RP01264) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Nucleoprotein Antibody (A18797) with a linear range of 3.12-200ng/mL.

Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

Immunoprecipitation - SARS-CoV-2 Nucleoprotein Rabbit pAb (A18797)

* For research use only. Not for therapeutic or diagnostic purposes.