제품 > 항체 > 다중클론항체(pAb)

SARS-CoV-2 ORF9b Rabbit pAb (A20260)

Review (1)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:SARS-CoV-2

ABclonal:SARS-CoV-2 ORF9b Rabbit pAb
ABclonal:Western blot - SARS-CoV-2 ORF9b Rabbit pAb (A20260)

Western blot analysis of extracts of normal 293T cells 293T transfected with ORF9b Protein, using SARS-CoV-2 ORF9b Rabbit pAb (A20260) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for SARS-CoV-2 ORF9b Rabbit pAb(A20260)

Overview

Product nameSARS-CoV-2 ORF9b Rabbit pAb
Catalog No.A20260
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is the causative agent of the COVID19 pandemic.?? SARS-CoV-2 and SARS-CoVshare many proteins common in other CoVs, including 4 majorstructural proteins (S, E, M, and N proteins) and 16 nonstructuralproteins (nsp1-16), they possess a unique set of proteins, namelyorf3a, 3b, 6, 7a, 7b, 8a, 8b, and 9b。The SARS-CoV-2 genome encodes for a small accessory protein termed Orf9b, ?? which targets the mitochondrial outer membrane protein TOM70 in infected cells.This 98-amino acid (aa) protein is encoded by analternative open reading frame (ORF) within the N gene and istranslated via a leaky scanning mechanism during translationCrystal structures of orf9b alone revealed a homodimericβ-strand-rich structure? with a hydro_x005fphobic central tunnel for lipid binding, consistent with the role oforf9b in the mature virion assembly
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-97 of coronavirus ORF9b (P0DTD2).
SequenceMDPKISEMHPALRLVDPQIQLAVTRMENAVGRDQNNVGPKVYPIILRLGSPLSLNMARKTLNSLEDKAFQLTPIAVQMTKLATTEELPDEFVVVTVK
Gene ID
Swiss Prot
Synonyms
Calculated MW11kDa
Observed MW12-14kDa
ReactivitySARS-CoV-2
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular location
Customer validation

(A549 cell lines)

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - SARS-CoV-2 ORF9b Rabbit pAb (A20260)}

    Western blot - SARS-CoV-2 ORF9b Rabbit pAb (A20260)

    Western blot analysis of extracts of normal 293T cells 293T transfected with ORF9b Protein, using SARS-CoV-2 ORF9b Rabbit pAb (A20260) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Submit an review for A20260

    Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

    Reviews List

    Publishing research using A20260? Please let us know so that we can cite the reference in this datasheet.