제품 > 항체 > 다중클론항체(pAb)

SIRT1 Rabbit pAb (A0230)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse testis, using SIRT1 Rabbit pAb (A0230) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - SIRT1 Rabbit pAb (A0230)

Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Rabbit pAb (A0230) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	SIRT1 Rabbit pAb
Catalog No.	A0230
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SIRT1 (NP_036370.2).
Sequence	ILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKL
Gene ID
Swiss Prot
Synonyms	SIR2; SIR2L1; SIR2alpha; SIRT1
Calculated MW	82kDa
Observed MW	100-120kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse testis
Cellular location	Cytoplasm, Mitochondrion, Nucleus, Nucleus, PML body.
Customer validation	(HeLa) WB(Homo sapiens, Mus musculus, Rattus norvegicus) IF(Sus scrofa, Mus musculus) IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

SIRT1 Rabbit pAb images

Western blot - SIRT1 Rabbit pAb (A0230)

Immunofluorescence - SIRT1 Rabbit pAb (A0230)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0230

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Reviews List

Product Protocols

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of Agricultural and Food Chemistry

Application:

IF:

5.279

Species:

Homo sapiens

PMID:

29886733

Title:

Improvement of Lipid and Glucose Metabolism by Capsiate in Palmitic Acid-Treated HepG2 Cells via Activation of the AMPK/SIRT1 Signaling Pathway.

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of Cellular Biochemistry

Application:

IF:

4.429

Species:

Mus musculus

PMID:

30335884

Title:

Reelin regulates male mouse reproductive capacity via the sertoli cells.

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.279

Species:

Mus musculus

PMID:

31075194

Title:

A Combination of Capsaicin and Capsiate Induces Browning in 3T3-L1 White Adipocytes via Activation of the PPARγ/ β3-AR Signaling Pathways.

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

PloS one

Application:

IF:

3.24

Species:

Homo sapiens

PMID:

31100089

Title:

Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes.

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Biochemical Pharmacology

Application:

IF:

5.858

Species:

Mus musculus

PMID:

26673543

Title:

The peroxisome proliferator-activated receptor γ agonist pioglitazone prevents NF-κB activation in cisplatin nephrotoxicity through the reduction of p65 acetylation via the AMPK-SIRT1/p300 pathway

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Brain Res Bull

Application:

IF:

4.077

Species:

Mus musculus

PMID:

32987101

Title:

Treadmill exercise mitigates neuroinflammation and increases BDNF via activation of SIRT1 signaling in a mouse model of T2DM

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Brain Res Bull

Application:

IF:

4.077

Species:

Mus musculus

PMID:

32871241

Title:

Myocardial infarction-induced anxiety-like behavior is associated with epigenetic alterations in the hippocampus of rat

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Genes Brain Behav

Application:

IF:

3.44

Species:

Mus musculus

PMID:

34355499

Title:

The role of SIRT1 in the basolateral amygdala in depression-like behaviors in mice

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Sci Rep

Application:

IF:

4.379

Species:

Mus musculus

PMID:

34045601

Title:

NUMB suppression by miR-9-5P enhances CD44+ prostate cancer stem cell growth and metastasis

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Sci Rep

Application:

IF:

4.379

Species:

Mus musculus

PMID:

34158600

Title:

Photobiomodulation has rejuvenating effects on aged bone marrow mesenchymal stem cells

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Frontiers in veterinary science

Application:

IF:

3.47

Species:

Sus scrofa

PMID:

35812864

Title:

Arginine Regulates Zygotic Genome Activation in Porcine Embryos Under Nutrition Restriction - PMC

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Neuroscience letters

Application:

IF:

3.19

Species:

Mus musculus

PMID:

38072030

Title:

AdipoRon reduces cisplatin-induced ototoxicity in hair cells: possible relation to the regulation of mitochondrial biogenesis

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Neural regeneration research

Application:

WB, IF

IF:

6.05

Species:

Rattus norvegicus

PMID:

37721302

Title:

Activation of G-protein-coupled receptor 39 reduces neuropathic pain in a rat model

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.4

Species:

Mus musculus

PMID:

38670408

Title:

Arjunolic acid ameliorates lipopolysaccharide-induced depressive behavior by inhibiting neuroinflammation via microglial SIRT1/AMPK/Notch1 signaling pathway

References for SIRT1 Rabbit pAb (A0230)

Product:

SIRT1 Rabbit pAb

Journal:

Frontiers in bioscience (Landmark edition)

Application:

IF:

3.1

Species:

Mus musculus

PMID:

38940051