제품 > 항체 > 다중클론항체(pAb)

SIRT1 Rabbit pAb (A11267)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using SIRT1 Rabbit pAb (A11267) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SIRT1 Rabbit pAb (A11267)

Immunohistochemistry analysis of paraffin-embedded human colon using SIRT1 Rabbit pAb (A11267) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	SIRT1 Rabbit pAb
Catalog No.	A11267
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 448-747 of human SIRT1 (NP_036370.2).
Sequence	VALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS
Gene ID
Swiss Prot
Synonyms	SIR2; SIR2L1; SIR2alpha; SIRT1
Calculated MW	82kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293F, HeLa
Cellular location	Cytoplasm, Mitochondrion, Nucleus, Nucleus, PML body
Customer validation	WB(Sus scrofa, Danio rerio, Rattus norvegicus, Mus musculus, Homo sapiens, Gallus gallus, Carassius gibelio, Bos taurus, Mus musculus, Other, Ctenopharyngodon idellus) IF(Homo sapiens, Mus musculus, Cherax quadricarinatus) IF(Homo sapiens, Mus musculus, Rattus norvegicus) ChIP(Mus musculus) IP(Homo sapiens) IHC(Mus musculus, Rattus norvegicus) ELISA(Rattus norvegicus)

Documents

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Research Area

SIRT1 Rabbit pAb images

Western blot - SIRT1 Rabbit pAb (A11267)

Immunohistochemistry - SIRT1 Rabbit pAb (A11267)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

BMC Genetics

Application:

IF:

2.797

Species:

Sus scrofa

PMID:

32171241

Title:

miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Autophagy

Application:

IF:

16.016

Species:

Danio rerio

PMID:

30205735

Title:

CERKL regulates autophagy via the NAD-dependent deacetylase SIRT1

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Cell Proliferation

Application:

IF:

6.831

Species:

Rattus norvegicus

PMID:

32020692

Title:

Resveratrol protects the integrity of alveolar epithelial barrier via SIRT1/PTEN/p-Akt pathway in methamphetamine-induced chronic lung injury

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

BMC Nephrology

Application:

IF:

2.388

Species:

Rattus norvegicus

PMID:

32398108

Title:

Kidney-targeted baicalin-lysozyme conjugate ameliorates renal fibrosis in rats with diabetic nephropathy induced by streptozotocin

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.81

Species:

Mus musculus

PMID:

32848753

Title:

Trimetazidine Inhibits Renal Tubular Epithelial Cells to Mesenchymal Transition in Diabetic Rats via Upregulation of Sirt1

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Bioengineered

Application:

IF:

3.269

Species:

Homo sapiens

PMID:

33323018

Title:

Catalpol ameliorates psoriasis-like phenotypes via SIRT1 mediated suppression of NF-κB and MAPKs signaling pathways

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Food Funct

Application:

IF:

5.39

Species:

Mus musculus

PMID:

33908429

Title:

Lycopene attenuates di(2-ethylhexyl) phthalate-induced mitophagy in spleen by regulating the sirtuin3-mediated pathway

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Pulm Pharmacol Ther

Application:

IF:

3.41

Species:

Mus musculus

PMID:

33887491

Title:

Gentiopicroside ameliorates ovalbumin-induced airway inflammation in a mouse model of allergic asthma via regulating SIRT1/NF-κB signaling pathway

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Environ Toxicol

Application:

IF:

4.11

Species:

Mus musculus

PMID:

34018660

Title:

Effects of manganese on microglia M1/M2 polarization and SIRT1-mediated transcription of STAT3-dependent genes in mouse

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Acta Biochim Biophys Sin (Shanghai)

Application:

IF:

3.848

Species:

Mus musculus

PMID:

34125142

Title:

Histidine ameliorates elastase-and lipopolysaccharide-induced lung inflammation by inhibiting the activation of the NLRP3 inflammasome

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Acta Pharmacol Sin

Application:

IF:

6.15

Species:

Mus musculus

PMID:

34853445

Title:

Dapagliflozin attenuates pressure overload-induced myocardial remodeling in mice via activating SIRT1 and inhibiting endoplasmic reticulum stress

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of applied toxicology : JAT

Application:

IF:

3.62

Species:

Mus musculus

PMID:

36008890

Title:

Liquiritin alleviates alpha‐naphthylisothiocyanate‐induced intrahepatic cholestasis through the Sirt1/FXR/Nrf2 pathway

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

IF:

2.65

Species:

Homo sapiens

PMID:

35911149

Title:

Arisaema heterophyllum Blume Monomer Stigmasterol Targets PPARγ and Inhibits the Viability and Tumorigenicity of Lung Adenocarcinoma Cells NCI-H1975 - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Toxicological sciences : an official journal of the Society of Toxicology

Application:

IF:

4.1

Species:

Homo sapiens

PMID:

34515779

Title:

Sodium Tanshinone IIA Sulfonate Attenuates Cigarette Smoke Extract-Induced Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis in Alveolar Epithelial Cells by Enhancing SIRT1 Pathway

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Mus musculus

PMID:

35784681

Title:

Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Pharmacological research

Application:

IF:

10.33

Species:

Mus musculus

PMID:

34826603

Title:

Overexpression of PDE4D in mouse liver is sufficient to trigger NAFLD and hypertension in a CD36-TGF-β1 pathway: therapeutic role of roflumilast

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

IF:

5.21

Species:

Homo sapiens

PMID:

36678523

Title:

Gold Nanoparticles Encapsulated Resveratrol as an Anti-Aging Agent to Delay Cataract Development - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Cancer gene therapy

Application:

IF:

5.85

Species:

Gallus gallus

PMID:

35902730

Title:

CYP2E1-dependent upregulation of SIRT7 is response to alcohol mediated metastasis in hepatocellular carcinoma

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Carassius gibelio

PMID:

36641865

Title:

Resveratrol attenuated oxidative stress and inflammatory and mitochondrial dysfunction induced by acute ammonia exposure in gibel carp (Carassius gibelio)

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Mus musculus

PMID:

37027942

Title:

SIRT1/FOXO3-mediated autophagy signaling involved in manganese-induced neuroinflammation in microglia

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of dairy science

Application:

IF:

4.22

Species:

Bos taurus

PMID:

37296051

Title:

A high-concentrate diet induces mitochondrial dysfunction by activating the MAPK signaling pathway in the mammary gland of dairy cows

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

World journal of stem cells

Application:

IF:

5.24

Species:

Rattus norvegicus

PMID:

37700818

Title:

Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Mus musculus

PMID:

37807259

Title:

Effect of Yeast-Derived Peptides on Skeletal Muscle Function and Exercise-Induced Fatigue in C2C12 Myotube Cells and ICR Mice

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Aging-us

Application:

WB, IF

IF:

5.955

Species:

Homo sapiens

PMID:

31881011

Title:

Hydrogen sulfide attenuates mitochondrial dysfunction-induced cellular senescence and apoptosis in alveolar epithelial cells by upregulating sirtuin 1.

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of Pineal Research

Application:

IF:

12.081

Species:

Homo sapiens, Mus musculus

PMID:

31469445

Title:

Melatonin/PGC1A/UCP1 promotes tumor slimming and represses tumor progression by initiating autophagy and lipid browning.

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Clinical and translational medicine

Application:

IF:

8.55

Species:

Homo sapiens, Mus musculus

PMID:

35872650

Title:

Metabolic rescue ameliorates mitochondrial encephalo-cardiomyopathy in murine and human iPSC models of Leigh syndrome - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Investigative ophthalmology & visual science

Application:

WB, IF

IF:

4.92

Species:

Mus musculus

PMID:

37099021

Title:

Neuroprotection of SRT2104 in Murine Ischemia/Reperfusion Injury Through the Enhancement of Sirt1-Mediated Deacetylation - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Experimental cell research

Application:

WB, IF

IF:

4.14

Species:

Homo sapiens

PMID:

36341908

Title:

Inhibition of 14-3-3 by K50 acetylation activates YAP1 to promote cholangiocarcinoma growth

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

6.63

Species:

Mus musculus

PMID:

38325588

Title:

Melatonin ameliorates retinal ganglion cell senescence and apoptosis in a SIRT1-dependent manner in an optic nerve injury model

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

BMC complementary medicine and therapies

Application:

IF:

2.83

Species:

Rattus norvegicus

PMID:

38331805

Title:

Astragalus polysaccharides attenuate rat aortic endothelial senescence via regulation of the SIRT-1/p53 signaling pathway - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Mus musculus

PMID:

38339136

Title:

Endothelial Mitochondria Transfer to Melanoma Induces M2-Type Macrophage Polarization and Promotes Tumor Growth by the Nrf2/HO-1-Mediated Pathway - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

6.1

Species:

Mus musculus,Other

PMID:

38259150

Title:

Studying Antifatigue Mechanism of Tyr-Pro-Leu-Pro in Exercise Mice Using Label-Free Proteomics

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Science Advances

Application:

WB, ChIP

IF:

14.957

Species:

Mus musculus

PMID:

32270032

Title:

B cell Sirt1 deacetylates histone and non-histone proteins for epigenetic modulation of AID expression and the antibody response

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Acta pharmaceutica Sinica. B

Application:

WB, IP

IF:

14.9

Species:

Homo sapiens

PMID:

36873184

Title:

SIRT1 activation synergizes with FXR agonism in hepatoprotection via governing nucleocytoplasmic shuttling and degradation of FXR

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Bioengineered

Application:

WB, IHC

IF:

6.832

Species:

Mus musculus

PMID:

33034242

Title:

Loganin inhibits macrophage M1 polarization and modulates sirt1/NF-B signaling pathway to attenuate ulcerative colitis

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Mediators of inflammation

Application:

IF:

4.529

Species:

Mus musculus

PMID:

35431653

Title:

Atractylenolide III Improves Mitochondrial Function and Protects Against Ulcerative Colitis by Activating AMPK/SIRT1/PGC-1

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Viruses

Application:

IF:

5.81

Species:

Cherax quadricarinatus

PMID:

36016356

Title:

A Deacetylase CqSIRT1 Promotes WSSV Infection by Binding to Viral Envelope Proteins in Cherax quadricarinatus - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

WB, IF

IF:

5.98

Species:

Rattus norvegicus

PMID:

35281931

Title:

Bilobalide Exerts Anti-Inflammatory Effects on Chondrocytes Through the AMPK/SIRT1/mTOR Pathway to Attenuate ACLT-Induced Post-Traumatic Osteoarthritis in Rats

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

WB, IHC, IF, ELISA

IF:

7.41

Species:

Rattus norvegicus

PMID:

36076592

Title:

Arctium lappa leaves based on network pharmacology and experimental validation attenuate atherosclerosis by targeting the AMPK-mediated PPARG/LXR

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

J Inflamm Res

Application:

IF:

4.631

Species:

Rattus norvegicus

PMID:

33623411

Title:

Securidaca inappendiculata-Derived Xanthones Protected Joints from Degradation in Male Rats with Collagen-Induced Arthritis by Regulating PPAR- Signaling

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Chemical biology & drug design

Application:

IF:

Species:

Homo sapiens,Mus musculus

PMID:

38570329

Title:

Icariin alleviates the apoptosis of chondrocytes in osteoarthritis through regulating SIRT1Nrf2HO1 signaling

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Placenta

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

38588616

Title:

Single cell RNA-sequencing reveals the cellular senescence of placental mesenchymal stem/stromal cell in preeclampsia

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Food chemistry

Application:

IF:

8.8

Species:

Ctenopharyngodon idellus

PMID:

38670026

Title:

Declined flesh quality resulting from niacin deficiency is associated with elevated glycolysis and impaired mitochondrial homeostasis in grass carp

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

38778385

Title:

Gouqi-derived nanovesicles (GqDNVs) inhibited dexamethasone-induced muscle atrophy associating with AMPK/SIRT1/PGC1 signaling pathway - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

6.1

Species:

Mus musculus

PMID:

38492240

Title:

Orientin Promotes Antioxidant Capacity, Mitochondrial Biogenesis, and Fiber Transformation in Skeletal Muscles through the AMPK Pathway

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Mus musculus

PMID:

38912487

Title:

Bazi Bushen capsule attenuates cardiac systolic injury via SIRT3/SOD2 pathway in high-fat diet-fed ovariectomized mice - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF:

5.8

Species:

Sus scrofa

PMID:

38705537

Title:

EGCG alleviates heat-stress-induced fat deposition by targeting HSP70 through activation of AMPK-SIRT1-PGC-1 in porcine subcutaneous preadipocytes

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Frontiers in molecular biosciences

Application:

IF:

Species:

Homo sapiens

PMID:

38894712

Title:

p53 deficiency mediates cisplatin resistance by upregulating RRM2 and crotonylation of RRM2K283 through the downregulation of SIRT7 - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

39000568

Title:

Quercetin Modulates Ferroptosis via the SIRT1/Nrf-2/HO-1 Pathway and Attenuates Cartilage Destruction in an Osteoarthritis Rat Model - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Journal of animal science

Application:

IF:

3.3

Species:

Gallus gallus

PMID:

35866893

Title:

Astragalus polysaccharide mitigates transport stress-induced hepatic metabolic stress via improving hepatic glucolipid metabolism in chicks

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Stem cell research & therapy

Application:

IF:

7.5

Species:

Mus musculus

PMID:

39285500

Title:

NQO1 promotes osteogenesis and suppresses angiogenesis in DPSCs via MAPK pathway modulation - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Foods (Basel, Switzerland)

Application:

IF:

5.2

Species:

Mus musculus

PMID:

39272560

Title:

Investigation of the Lipid-Lowering Activity and Mechanism of Three Extracts from Astragalus membranaceus, Hippophae rhamnoides L., and Taraxacum mongolicum Hand. Mazz Based on Network Pharmacology and In Vitro and In Vivo Experiments - PMC

References for SIRT1 Rabbit pAb (A11267)

Product:

SIRT1 Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

Species:

Homo sapiens

PMID:

35387262

Title: