제품 > 항체 > 다중클론항체(pAb)

SMO Rabbit pAb (A3274)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of extracts of various cell lines, using SMO antibody (A3274) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Western blot analysis of extracts of Mouse brain, using SMO antibody (A3274) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review

Overview

Product name	SMO Rabbit pAb
Catalog No.	A3274
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 28-233 of human SMO (NP_005622.1).
Sequence	RGAASSGNATGPGPRSAGGSARRSAAVTGPPPPLSHCGRAAPCEPLRYNVCLGSVLPYGATSTLLAGDSDSQEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDRFPEGCTNEVQNIKFNSSGQCEVPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSY
Gene ID
Swiss Prot
Synonyms	Gx; CRJS; PHLS; SMOH; FZD11
Calculated MW	86kDa
Observed MW	86kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, 293T, Mouse brain
Cellular location	Cell projection, Membrane, Multi-pass membrane protein, cilium
Customer validation	(Mouse liver) WB(Mus musculus, Drosophila, Rattus norvegicus, Homo sapiens, Drosophila melanogaster) IP(Drosophila melanogaster)

Documents

Certificate of Compliance

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Lot number

Research Area

SMO Rabbit pAb images

Western blot - SMO Rabbit pAb (A3274)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Oncogenesis

Application:

IF:

7.485

Species:

Mus musculus

PMID:

30563983

Title:

miR-100 maintains phenotype of tumor-associated macrophages by targeting mTOR to promote tumor metastasis via Stat5a/IL-1ra pathway in mouse breast cancer.

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

3.575

Species:

Drosophila

PMID:

31010679

Title:

HERC4 exerts an anti-tumor role through destabilizing the oncoprotein Smo.

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

International journal of biological sciences

Application:

IF:

6.58

Species:

Mus musculus

PMID:

31360105

Title:

Tripartite Motif 8 Deficiency Relieves Hepatic Ischaemia/reperfusion Injury via TAK1-dependent Signalling Pathways.

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Toxicology letters

Application:

IF:

4.372

Species:

Rattus norvegicus

PMID:

31181250

Title:

Targeting the RAS axis alleviates silicotic fibrosis and Ang II-induced myofibroblast differentiation via inhibition of the hedgehog signaling pathway.

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Theranostics

Application:

IF:

11.556

Species:

Rattus norvegicus

PMID:

32042332

Title:

Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Nature Communications

Application:

IF:

14.919

Species:

Mus musculus

PMID:

32332736

Title:

Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Oncotarget

Application:

IF:

3.765

Species:

Homo sapiens

PMID:

27105509

Title:

Gli-1/PI3K/AKT/NF-kB pathway mediates resistance to radiation and is a target for reversion of responses in refractory acute myeloid leukemia cells.

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Cell Death Dis

Application:

IF:

8.469

Species:

Mus musculus

PMID:

32826873

Title:

Protein phosphatase 4 promotes Hedgehog signaling through dephosphorylation of Suppressor of fused

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Diabetes Res Clin Pract

Application:

IF:

5.602

Species:

Rattus norvegicus

PMID:

34742786

Title:

FOXM1 accelerates wound healing in diabetic foot ulcer by inducing M2 macrophage polarization through a mechanism involving SEMA3C/NRP2/Hedgehog …

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Bone

Application:

IF:

4.62

Species:

Mus musculus

PMID:

36152943

Title:

MiR-1 is a critical regulator of chondrocyte proliferation and hypertrophy by inhibiting Indian hedgehog pathway during postnatal endochondral ossification in miR-1 …

References for SMO Rabbit pAb (A3274)

Product:

SMO Rabbit pAb

Journal:

Cell Death Differ

Application:

WB, IP

IF:

12.067

Species:

Drosophila melanogaster

PMID: