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SOX2 Rabbit pAb (A0561)

Reviews (2)Publications (27) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SOX2 Rabbit pAb (A0561)

Western blot analysis of lysates from MCF7 cells, using SOX2 Rabbit pAb (A0561) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - SOX2 Rabbit pAb (A0561)

Western blot analysis of various lysates using SOX2 Rabbit pAb (A0561) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - SOX2 Rabbit pAb (A0561)

Western blot analysis of lysates from Mouse brain, using SOX2 Rabbit pAb (A0561) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - SOX2 Rabbit pAb (A0561)

Western blot analysis of various lysates using SOX2 Rabbit pAb (A0561) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - SOX2 Rabbit pAb (A0561)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using SOX2 Rabbit pAb (A0561) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SOX2 Rabbit pAb (A0561)

Immunohistochemistry analysis of paraffin-embedded Mouse embryos using SOX2 Rabbit pAb (A0561) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SOX2 Rabbit pAb (A0561)

Immunohistochemistry analysis of paraffin-embedded Mouse embryos using SOX2 Rabbit pAb (A0561) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - SOX2 Rabbit pAb (A0561)

Immunofluorescence analysis of F9 cells using SOX2 Rabbit pAb (A0561) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SOX2 Rabbit pAb (A0561)

Immunoprecipitation analysis of 200 μg extracts of C6 cells using 3 μg SOX2 antibody (A0561). Western blot was performed from the immunoprecipitate using SOX2 antibody (A0561) at a dilution of 1:500.

Review
ABclonal: review for SOX2 Rabbit pAb(A0561)
Review
ABclonal: review for SOX2 Rabbit pAb(A0561)

Overview

Product nameSOX2 Rabbit pAb
Catalog No.A0561
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOX2 (NP_003097.1).
SequenceMYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMA
Gene ID
Swiss Prot
SynonymsANOP3; MCOPS3; SOX2
Calculated MW34kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, MCF7, Mouse lung, Rat brain
Cellular locationNucleus.
Customer validation

(neural progenitors , mouse brain)

IP(Homo sapiens)

WB(Homo sapiens, Mus musculus)

IHC(Mus musculus, Homo sapiens)

IF(Gallus gallus, Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

IP(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - SOX2 Rabbit pAb (A0561)}

Western blot - SOX2 Rabbit pAb (A0561)

Western blot analysis of lysates from MCF7 cells, using SOX2 Rabbit pAb (A0561) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - SOX2 Rabbit pAb (A0561)}

Western blot - SOX2 Rabbit pAb (A0561)

Western blot analysis of various lysates using SOX2 Rabbit pAb (A0561) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - SOX2 Rabbit pAb (A0561)}

Western blot - SOX2 Rabbit pAb (A0561)

Western blot analysis of lysates from Mouse brain, using SOX2 Rabbit pAb (A0561) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - SOX2 Rabbit pAb (A0561)}

Western blot - SOX2 Rabbit pAb (A0561)

Western blot analysis of various lysates using SOX2 Rabbit pAb (A0561) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - SOX2 Rabbit pAb (A0561)}

Immunohistochemistry - SOX2 Rabbit pAb (A0561)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using SOX2 Rabbit pAb (A0561) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SOX2 Rabbit pAb (A0561)}

Immunohistochemistry - SOX2 Rabbit pAb (A0561)

Immunohistochemistry analysis of paraffin-embedded Mouse embryos using SOX2 Rabbit pAb (A0561) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SOX2 Rabbit pAb (A0561)}

Immunohistochemistry - SOX2 Rabbit pAb (A0561)

Immunohistochemistry analysis of paraffin-embedded Mouse embryos using SOX2 Rabbit pAb (A0561) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - SOX2 Rabbit pAb (A0561)}

Immunofluorescence - SOX2 Rabbit pAb (A0561)

Immunofluorescence analysis of F9 cells using SOX2 Rabbit pAb (A0561) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - SOX2 Rabbit pAb (A0561)}

Immunoprecipitation - SOX2 Rabbit pAb (A0561)

Immunoprecipitation analysis of 200 μg extracts of C6 cells using 3 μg SOX2 antibody (A0561). Western blot was performed from the immunoprecipitate using SOX2 antibody (A0561) at a dilution of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)