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SOX9 Rabbit mAb (A19710)

Publications (34) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SOX9 Rabbit mAb (A19710)

Western blot analysis of various lysates using SOX9 Rabbit mAb (A19710) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human colon using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human pancreas using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human pancreatic ductal carcinoma using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human stomach using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - SOX9 Rabbit mAb (A19710)

Confocal imaging of paraffin-embedded Mouse testis using SOX9 Rabbit mAb (A19710, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - SOX9 Rabbit mAb (A19710)

Confocal imaging of paraffin-embedded Rat testis using SOX9 Rabbit mAb (A19710, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - SOX9 Rabbit mAb (A19710)

Confocal imaging of HCT 116 cells using SOX9 Rabbit mAb (A19710, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - SOX9 Rabbit mAb (A19710)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SOX9 antibody (A19710). Western blot was performed from the immunoprecipitate using SOX9 antibody (A19710) at a dilution of 1:1000.

Overview

Product nameSOX9 Rabbit mAb
Catalog No.A19710
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0190
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 169-300 of human SOX9 (P48436).
SequenceHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPP
Gene ID
Swiss Prot
SynonymsCMD1; SRA1; CMPD1; SRXX2; SRXY10; SOX9
Calculated MW56kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:200 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHep G2, HeLa
Cellular locationNucleus.
Customer validation

IHC(Mus musculus)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus, Bos taurus)

IHC(Mus musculus, Rattus norvegicus)

IF(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Mus musculus)

PCR(Homo sapiens,Mus musculus)

RT-PCR(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - SOX9 Rabbit mAb (A19710)}

Western blot - SOX9 Rabbit mAb (A19710)

Western blot analysis of various lysates using SOX9 Rabbit mAb (A19710) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)}

Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human colon using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)}

Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)}

Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human pancreas using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)}

Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human pancreatic ductal carcinoma using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SOX9 Rabbit mAb (A19710)}

Immunohistochemistry - SOX9 Rabbit mAb (A19710)

Immunohistochemistry analysis of paraffin-embedded Human stomach using SOX9 Rabbit mAb (A19710) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - SOX9 Rabbit mAb (A19710)}

Immunofluorescence - SOX9 Rabbit mAb (A19710)

Confocal imaging of paraffin-embedded Mouse testis using SOX9 Rabbit mAb (A19710, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - SOX9 Rabbit mAb (A19710)}

Immunofluorescence - SOX9 Rabbit mAb (A19710)

Confocal imaging of paraffin-embedded Rat testis using SOX9 Rabbit mAb (A19710, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - SOX9 Rabbit mAb (A19710)}

Immunofluorescence - SOX9 Rabbit mAb (A19710)

Confocal imaging of HCT 116 cells using SOX9 Rabbit mAb (A19710, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunoprecipitation - SOX9 Rabbit mAb (A19710)}

Immunoprecipitation - SOX9 Rabbit mAb (A19710)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg SOX9 antibody (A19710). Western blot was performed from the immunoprecipitate using SOX9 antibody (A19710) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19710? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

SOX9 Rabbit pAb
A2479

ELISAWBIHC-P

Reactivity:Human, Mouse, Rat

SOX9 Rabbit pAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)