제품 > 항체 > 다중클론항체(pAb)

SP1 Rabbit pAb (A14662)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse heart, using SP1 Rabbit pAb (A14662) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SP1 Rabbit pAb (A14662)

Immunohistochemistry analysis of paraffin-embedded Rat liver using SP1 Rabbit pAb (A14662) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen using SP1 Rabbit pAb (A14662) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using SP1 Rabbit pAb (A14662) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - SP1 Rabbit pAb (A14662)

Immunofluorescence analysis of NIH/3T3 cells using SP1 Rabbit pAb (A14662) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	SP1 Rabbit pAb
Catalog No.	A14662
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 420-620 of human SP1 (NP_612482.2).
Sequence	LSGQTFTTQAISQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCPYCKDSEGRGSGDP
Gene ID
Swiss Prot
Synonyms	SP1
Calculated MW	81kDa
Observed MW	90kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse heart
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Sus scrofa, Rattus norvegicus, Homo sapiens, Capra hircus) ChIP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

SP1 Rabbit pAb images

Western blot - SP1 Rabbit pAb (A14662)

Immunohistochemistry - SP1 Rabbit pAb (A14662)

Immunofluorescence - SP1 Rabbit pAb (A14662)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for SP1 Rabbit pAb (A14662)

Product:

SP1 Rabbit pAb

Journal:

J Virol

Application:

IF:

5.103

Species:

Sus scrofa

PMID:

34232065

Title:

Porcine epidemic diarrhea virus inhibits HDAC1 expression to facilitate its replication via binding of its nucleocapsid protein to host transcription factor Sp1

References for SP1 Rabbit pAb (A14662)

Product:

SP1 Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Rattus norvegicus

PMID:

37673870

Title:

FNDC5 inhibits autophagy of bone marrow mesenchymal stem cells and promotes their survival after transplantation by downregulating Sp1 - PMC

References for SP1 Rabbit pAb (A14662)

Product:

SP1 Rabbit pAb

Journal:

Pathogens (Basel, Switzerland)

Application:

IF:

4.53

Species:

Homo sapiens

PMID:

36014967

Title:

Helicobacter pylori CagA Protein Regulating the Biological Characteristics of Gastric Cancer through the miR-155-5p/SMAD2/SP1 axis - PMC

References for SP1 Rabbit pAb (A14662)

Product:

SP1 Rabbit pAb

Journal:

iScience

Application:

ChIP

IF:

6.1

Species:

Homo sapiens

PMID:

37009226

Title:

Copper overload impairs hematopoietic stem and progenitor cell proliferation via prompting HSF1/SP1 aggregation and the subsequently downregulating FOXM1-Cytoskeleton axis - PMC

References for SP1 Rabbit pAb (A14662)

Product:

SP1 Rabbit pAb

Journal:

Journal of advanced research

Application:

IF:

12.82

Species:

Homo sapiens

PMID:

37182685

Title:

PIBF1 regulates multiple gene expression via impeding long-range chromatin interaction to drive the malignant transformation of HPV16 integration epithelial

References for SP1 Rabbit pAb (A14662)

Product:

SP1 Rabbit pAb

Journal:

Mol Cell Endocrinol

Application:

IF:

4.1

Species:

Homo sapiens

PMID:

33662476