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SQSTM1/p62 Rabbit pAb (A7758)

Reviews (8)Publications (104) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SQSTM1/p62 Rabbit pAb (A7758)

Western blot analysis of various lysates using SQSTM1/p62 Rabbit pAb (A7758) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of paraffin-embedded Rat stomach using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)

Immunofluorescence analysis of HeLa cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)

Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)
Review
ABclonal: review for SQSTM1/p62 Rabbit pAb(A7758)

Overview

Product nameSQSTM1/p62 Rabbit pAb
Catalog No.A7758
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_003891.1).
SequenceMASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Gene ID
Swiss Prot
Synonymsp60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3; SQSTM1/p62
Calculated MW48kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA549, HeLa, 293T, PC-12
Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
Customer validation

(U-87MG cells, Human lung cancer cell, PANC-1, Squamous cell carcinoma, A549, Mouse cells, HepG2, Human Isikawa )

WB(Sus scrofa, Homo sapiens, Rattus norvegicus, Mus musculus, Danio rerio, Spodoptera frugiperda, Ctenopharyngodon idellus, Gallus gallus, Ctenopharyngodon idella, Apostichopus japonicus, Homo sapiens, Mus musculus, Horabagrus nigricollaris, Bombyx mori Linnaeus)

IP(Homo sapiens)

IHC(Rattus norvegicus, Ctenopharyngodon idellus)

IP(Homo sapiens)

IF(Mus musculus, Homo sapiens, Other)

IHC(Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

WB(Ctenopharyngodon idellus, Mus musculus)

Other(Rattus norvegicus)

qPCR(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - SQSTM1/p62 Rabbit pAb (A7758)}

Western blot - SQSTM1/p62 Rabbit pAb (A7758)

Western blot analysis of various lysates using SQSTM1/p62 Rabbit pAb (A7758) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)}

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)}

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)}

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)}

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A7758)

Immunohistochemistry analysis of paraffin-embedded Rat stomach using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)}

Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)

Immunofluorescence analysis of HeLa cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)}

Immunofluorescence - SQSTM1/p62 Rabbit pAb (A7758)

Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit pAb (A7758) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

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WBIHC-PIF/ICCIPELISA

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ELISAWB

Reactivity:Human, Mouse, Rat

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Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)