제품 > 항체 > 다중클론항체(pAb)

STAT3 Rabbit pAb (A11216)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using STAT3 Rabbit pAb (A11216) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A11216)

Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (A11216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (A11216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (A11216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	STAT3 Rabbit pAb
Catalog No.	A11216
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 600-700 of human STAT3 (NP_003141.2).
Sequence	TKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG
Gene ID
Swiss Prot
Synonyms	APRF; HIES; ADMIO; ADMIO1
Calculated MW	88kDa
Observed MW	79kDa/86kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	A-549, HeLa, NIH/3T3, RAW264.7, C6
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Homo sapiens, Mus musculus) IF(Mus musculus)

Documents

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Research Area

STAT3 Rabbit pAb images

Western blot - STAT3 Rabbit pAb (A11216)

Immunofluorescence - STAT3 Rabbit pAb (A11216)

Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (A11216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - STAT3 Rabbit pAb (A11216)

Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (A11216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - STAT3 Rabbit pAb (A11216)

Immunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (A11216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for STAT3 Rabbit pAb (A11216)

Product:

STAT3 Rabbit pAb

Journal:

Life (Basel)

Application:

IF:

Species:

Homo sapiens

PMID:

34063134

Title:

Interleukin-6 and Interleukin-8 Regulate STAT3 Activation Migration/Invasion and EMT in Chrysophanol-Treated Oral Cancer Cell Lines

References for STAT3 Rabbit pAb (A11216)

Product:

STAT3 Rabbit pAb

Journal:

Oncol Lett

Application:

IF:

2.967

Species:

Homo sapiens

PMID:

33552262

Title:

L-fucose ameliorates the carcinogenic properties of Fusobacterium nucleatum in colorectal cancer

References for STAT3 Rabbit pAb (A11216)

Product:

STAT3 Rabbit pAb

Journal:

Stem Cell Res Ther

Application:

IF:

6.83

Species:

Homo sapiens

PMID:

32736659

Title:

Hepatocyte growth factor (HGF) and stem cell factor (SCF) maintained the stemness of human bone marrow mesenchymal stem cells (hBMSCs) during long-term expansion by preserving mitochondrial function via the PI3K/AKT, ERK1/2, and STAT3 signaling pathways

References for STAT3 Rabbit pAb (A11216)

Product:

STAT3 Rabbit pAb

Journal:

Chinese medicine

Application:

IF:

4.54

Species:

Mus musculus

PMID:

37221600

Title:

TMT proteomics analysis reveals the mechanism of bleomycin-induced pulmonary fibrosis and effects of Ginseng honeysuckle superfine powdered tea - PMC

References for STAT3 Rabbit pAb (A11216)

Product:

STAT3 Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Homo sapiens

PMID:

38253527

Title:

EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses - PMC

References for STAT3 Rabbit pAb (A11216)

Product:

STAT3 Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

7.31

Species:

Mus musculus

PMID:

35770048