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STAT3 Rabbit pAb (A1192)

Reviews (3)Publications (73) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - STAT3 Rabbit pAb (A1192)

Western blot analysis of various lysates, using STAT3 Rabbit pAb (A1192) at 1:2500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - STAT3 Rabbit pAb (A1192)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - STAT3 Rabbit pAb (A1192)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - STAT3 Rabbit pAb (A1192)

Immunohistochemistry analysis of paraffin-embedded Rat heart using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - STAT3 Rabbit pAb (A1192)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (A1192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (A1192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (A1192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for STAT3 Rabbit pAb(A1192)
Review
ABclonal: review for STAT3 Rabbit pAb(A1192)
Review
ABclonal: review for STAT3 Rabbit pAb(A1192)

Overview

Product nameSTAT3 Rabbit pAb
Catalog No.A1192
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-700 of human STAT3 (NP_003141.2).
SequenceTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG
Gene ID
Swiss Prot
SynonymsAPRF; HIES; ADMIO; ADMIO1; STAT3
Calculated MW88kDa
Observed MW86kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, HeLa
Cellular locationCytoplasm, Nucleus.
Customer validation

(Human breast cancer, PK15)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Mus musculus, Sus scrofa, Rattus norvegicus)

IHC(Gallus gallus, Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens, Mus musculus)

WB(Homo sapiens)

Co-IP(Rattus norvegicus)

IF(Mus musculus)

Biochemical detection(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - STAT3 Rabbit pAb (A1192)}

Western blot - STAT3 Rabbit pAb (A1192)

Western blot analysis of various lysates, using STAT3 Rabbit pAb (A1192) at 1:2500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - STAT3 Rabbit pAb (A1192)}

Immunohistochemistry - STAT3 Rabbit pAb (A1192)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - STAT3 Rabbit pAb (A1192)}

Immunohistochemistry - STAT3 Rabbit pAb (A1192)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - STAT3 Rabbit pAb (A1192)}

Immunohistochemistry - STAT3 Rabbit pAb (A1192)

Immunohistochemistry analysis of paraffin-embedded Rat heart using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - STAT3 Rabbit pAb (A1192)}

Immunohistochemistry - STAT3 Rabbit pAb (A1192)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)}

Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (A1192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)}

Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (A1192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)}

Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (A1192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)}

Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)}

Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of PC-12 cells using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - STAT3 Rabbit pAb (A1192)}

Immunofluorescence - STAT3 Rabbit pAb (A1192)

Immunofluorescence analysis of U2OS cells using STAT3 Rabbit pAb (A1192) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (8)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)