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SUPT16H (SPT16) Rabbit pAb (A18696)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SUPT16H (SPT16) Rabbit pAb (A18696)

Western blot analysis of various lysates using SUPT16H (SPT16) Rabbit pAb (A18696) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - SUPT16H (SPT16) Rabbit pAb (A18696)

Immunofluorescence analysis of U2OS cells using SUPT16H (SPT16) Rabbit pAb (A18696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SUPT16H (SPT16) Rabbit pAb (A18696)

Immunofluorescence analysis of NIH/3T3 cells using SUPT16H (SPT16) Rabbit pAb (A18696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSUPT16H (SPT16) Rabbit pAb
Catalog No.A18696
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 520-695 of human SUPT16H (SPT16) (NP_009123.1).
SequenceKEPHIREMKIYIDKKYETVIMPVFGIATPFHIATIKNISMSVEGDYTYLRINFYCPGSALGRNEGNIFPNPEATFVKEITYRASNIKAPGEQTVPALNLQNAFRIIKEVQKRYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGD
Gene ID
Swiss Prot
SynonymsCDC68; SPT16; NEDDFAC; FACTP140; SPT16/CDC68; SUPT16H (SPT16)
Calculated MW120kDa
Observed MW140kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHCT 116, C6
Cellular locationnucleoplasm, nucleus.

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ABclonal:Western blot - SUPT16H (SPT16) Rabbit pAb (A18696)}

Western blot - SUPT16H (SPT16) Rabbit pAb (A18696)

Western blot analysis of various lysates using SUPT16H (SPT16) Rabbit pAb (A18696) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - SUPT16H (SPT16) Rabbit pAb (A18696)}

Immunofluorescence - SUPT16H (SPT16) Rabbit pAb (A18696)

Immunofluorescence analysis of U2OS cells using SUPT16H (SPT16) Rabbit pAb (A18696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SUPT16H (SPT16) Rabbit pAb (A18696)}

Immunofluorescence - SUPT16H (SPT16) Rabbit pAb (A18696)

Immunofluorescence analysis of NIH/3T3 cells using SUPT16H (SPT16) Rabbit pAb (A18696) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)