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Serotonin transporter Rabbit pAb (A14171)

Publications (9) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Serotonin transporter Rabbit pAb (A14171)

Western blot analysis of lysates from Rat lung, using Serotonin transporter Rabbit pAb (A14171) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171)

Immunohistochemistry analysis of paraffin-embedded Human colon using Serotonin transporter Rabbit pAb (A14171) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameSerotonin transporter Rabbit pAb
Catalog No.A14171
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake. There have been conflicting results in the literature about the possible effect, if any, that this polymorphism may play in behavior and depression.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-84 of human Serotonin transporter (NP_001036.1).
SequenceMETTPLNSQKQLSACEDGEDCQENGVLQKVVPTPGDKVESGQISNGYSAVPSPGAGDDTRHSIPATTTTLVAELHQGERETWGK
Gene ID
Swiss Prot
SynonymsHTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR; Serotonin transporter
Calculated MW70kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat lung
Cellular locationCell membrane, Endomembrane system, Endosome membrane, Multi-pass membrane protein.
Customer validation

WB(Mus musculus, Zea mays, Rattus norvegicus)

IHC(Mus musculus)

IF(Mus musculus)

IF(Zea mays)

Documents

Certificate of Compliance

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ABclonal:Western blot - Serotonin transporter Rabbit pAb (A14171)}

Western blot - Serotonin transporter Rabbit pAb (A14171)

Western blot analysis of lysates from Rat lung, using Serotonin transporter Rabbit pAb (A14171) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171)}

Immunohistochemistry - Serotonin transporter Rabbit pAb (A14171)

Immunohistochemistry analysis of paraffin-embedded Human colon using Serotonin transporter Rabbit pAb (A14171) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)