제품 > 항체 > 단일클론항체(mAb)

α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Reviews (3)Publications (157) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Western blot analysis of various lysates, using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Western blot analysis of various lysates using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for α-Smooth Muscle Actin (ACTA2) Rabbit mAb(A17910)
Review
ABclonal: review for α-Smooth Muscle Actin (ACTA2) Rabbit mAb(A17910)
Review
ABclonal: review for α-Smooth Muscle Actin (ACTA2) Rabbit mAb(A17910)

Overview

Product nameα-Smooth Muscle Actin (ACTA2) Rabbit mAb
Catalog No.A17910
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0298
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human α-Smooth Muscle Actin (ACTA2) (P62736).
SequenceMCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAP
Gene ID
Swiss Prot
SynonymsACTSA; α-Smooth Muscle Actin (ACTA2)
Calculated MW42kDa
Observed MW42kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:10000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRD, LNCaP, Mouse large intestine, Mouse brain, Rat lung
Cellular locationCytoplasm, cytoskeleton.
Customer validation

(Mouse MRC-9 cells, renal tubular epithelial cells, 293T KYM-1 SKOV-3)

IHC(Mus musculus, Homo sapiens, Rattus norvegicus, Mus musculus)

WB(Zea mays, Mus musculus, Oryza sativa, Rattus norvegicus, Homo sapiens, N. benthamiana, Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum, Solanum lycopersicum, Drosophila melanogaster, Mus musculus, Other, Rattus norvegicus)

IF(Homo sapiens, Sus scrofa, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens,Rattus norvegicus, Mus musculus)

qPCR(Arabidopsis thaliana)

IF(Homo sapiens)

IF(Homo sapiens, Mus musculus, Mus musculus, Rattus norvegicus)

IHC(Mus musculus)

WB(Homo sapiens, Mus musculus)

Co-IP(Oryza sativa)

Other(Mus musculus)

ELISA(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)}

Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Western blot analysis of various lysates, using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)}

Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910)

Western blot analysis of various lysates using α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A17910) at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A17910

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Publishing research using A17910? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

α-Smooth Muscle Actin (ACTA2) Rabbit pAb
A7248

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

α-Smooth Muscle Actin (ACTA2) Rabbit pAb

α-Smooth Muscle Actin (ACTA2) Rabbit pAb
A1011

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

α-Smooth Muscle Actin (ACTA2) Rabbit pAb

ELISA 키트 (1)

Human α2-Smooth muscle actin (α2-SMA) ELISA Kit
RK08013

Human α2-Smooth muscle actin (α2-SMA) ELISA Kit

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)