Description
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It can catalyze the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. T3 DNA Ligase will join blunt end and cohesive end termini. It and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and close the gaps in these DNA substrates. In addition, T3 DNA Ligase is more tolerant to NaCl than T4 DNA Ligase (2-fold).
As with T4 DNA Ligase , addition of PEG 6000 to the T3 DNA Ligase reaction system can improve the ligation efficiency of the blunt end. 1X T4 DNA Ligase Reaction Buffer can be used in some experiments where PEG 6000 is not available, but the activity of T3 DNA Ligase is reduced to 1/10. In applications where high concentrations of NaCl need to be maintained, we recommend the use of reaction buffers without PEG 6000.
Product Features
- High salt tolerance.
- Catalyze the formation of phosphodiester bonds at flat and sticky ends.
- Repair double-stranded DNA, RNA, DNA/RNA hybrid strand cutting.
- Strict batch performance and stability quality control.
Applications
Cloning of restriction fragments.
Joining linkers and adapters to blunt-ended DNA.
Circularization of linear DNA.
Storage
-20°C
Product Components
| Components | 100, 000 U | 750, 000 U |
| T3 DNA Ligase | 34 μL | 250 μL |
| 2X Universal DNA Ligase Buffer | 1 mL | 1 mL X 3 |
Product Data
Using double-stranded linear DNA molecules with one sticky end (HindIII digestion) of 700bp and 1400bp, respectively, ABclonal T3 DNA Ligase (1μL) and T3 DNA Ligase (N**) were added to test the enzyme activity tolerance under different salt concentrations (20μL ligation system), and the ligation products were detected by gel electrophoresis. The results showed that with the increase of NaCl concentration in the ligation system, the performance of ABclonal T3 DNA Ligase was not affected. The ligation efficiency of Abclonal T3 DNA ligase at 300mM NaCl concentration was comparable to that of competitors.
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