Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.
Product Features
- Application: It can catalyze the formation of phosphodilipid bond at flat end and sticky end, and repair double-stranded DNA, RNA, DNA/RNA hybrid strand cutting;
- Suitable for various types of samples: gDNA/FFPE DNA/cfDNA/ChIP DNA;
- Suitable for many types of segment connection: compatible with Illumina platform Y connector and MGI platform Bubble connector segment;
- Strict quality control: strict batch performance and stability quality control;
Workflow Chart
Storage
-20°C
Product Components
| Components | 80, 000 U | 400, 000 U |
| T4 DNA Ligase (2, 000, 000 U/mL) | 40 μL | 200 μL |
| 10X T4 DNA ligase Reaction Buffer | 500 μL | 500 μL |
Product Data
- ABclonal T4 DNA Ligase has extreme purity and excellent DNA ligation activity
A. SDS-Page analysis of different amounts of ABclonal T4 DNA ligase.
B. Analysis of ligation activity of ABclonal T4 DNA ligase at different times (2000 U ABclonal T4 DNA ligase ligated 1 ug HindIII-digested Lambda DNA fragment).
Documents
###RM21500|||10X T4 DNA ligase Reaction Buffer###RM20108)
