Description
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of template and primer. This enzyme has a 3'→5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5'→3' exonuclease function.
It is applicable to 3' overhang removal to form blunt ends, 5' overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis.
Workflow Chart
Storage
-20°C
Product Components
| Components | Concentration | 200 U | 1, 000 U |
| T4 DNA Polymerase (5, 000 U/ml) | 5, 000 U/mL | 40 μL | 200 μL |
| 10X ABuffer B | 10X | 1.25 mL | 1.25 mL X 2 |
Documents
###RM21302|||10X ABuffer B###RM20126)
