Description
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It can catalyze the formation of a phosphodiester bond between adjacent 5' phosphate and 3' hydroxyl termini in double-stranded DNA. T7 DNA ligase can catalyze ligation of cohesive ends and repair nicks, but not blunt end ligation. This makes a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined .
Product Features
- Only used to connect sticky ends.
- Nick-sealing in dsDNA.
- Strict batch performance and stability quality control.
Applications
Cloning of restriction fragments.
Joining linkers and adapters to blunt-ended DNA.
Nick-sealing in dsDNA
Circularization of linear DNA
Site-directed mutagenesis
Storage
-20°C
Product Components
| Components | 100, 000 U | 750, 000 U |
| T7 DNA Ligase | 34 μL | 250 μL |
| 2X Universal DNA Ligase Buffer | 1 mL | 1 mL X 3 |
Product Data
Using double-stranded linear DNA molecules with one sticky end (HindIII digestion) of 700bp and 1400bp, respectively, ABclonal T7 DNA Ligase and T3 DNA Ligase (N**) were added to test the Ligation efficiency under different enzyme content (20μL ligation system), and the ligation products were detected by gel electrophoresis. The results showed that ABclonal T7 DNA Ligase had high ligation efficiency in different enzyme contents.
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