Description
T7 Endonuclease I (T7 Endo I, T7EI)，can recognize and cleave non-perfectly mismatched DNA, cruciform DNA structures, holliday structures or junctions and Heteroduplex DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ end to the mismatch.
Applications
Mutation detection caused by gene editing;EMS mutagenic mutant detection in Tilling technology
Storage
-20°C
Product Components
| Components | Concentration | 250 U | 1250 U |
| T7 Endonuclease I | 10, 000 U/mL | 25 μL | 125 μL |
| 10X Buffer CutB | 10X | 1.25 mL | 1.25 mL |
Documents
