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TCP1 beta/CCT2 Rabbit pAb (A6546)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using TCP1 beta/CCT2 Rabbit pAb (A6546) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunofluorescence - TCP1 beta/CCT2 Rabbit pAb (A6546)

Immunofluorescence analysis of HeLa cells using TCP1 beta/CCT2 Rabbit pAb (A6546). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - TCP1 beta/CCT2 Rabbit pAb (A6546)

Immunoprecipitation analysis of 200 μg extracts of A-549 cells, using 3 μg TCP1 beta/TCP1 beta/CCT2 antibody (A6546). Western blot was performed from the immunoprecipitate using TCP1 beta/TCP1 beta/CCT2 antibody (A6546) at a dilution of 1:1000.

Overview

Product name	TCP1 beta/CCT2 Rabbit pAb
Catalog No.	A6546
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human TCP1 beta/TCP1 beta/CCT2 (NP_006422.1).
Sequence	GGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC
Gene ID
Swiss Prot
Synonyms	CCTB; 99D8.1; PRO1633; CCT-beta; HEL-S-100n; TCP-1-beta; TCP1 beta/CCT2
Calculated MW	57kDa
Observed MW	57kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	MCF7, SKOV3, A-549, HT-29, Mouse spleen, Mouse ovary, Mouse heart
Cellular location	Cytoplasm.
Customer validation	WB(Rattus norvegicus, Homo sapiens, Mus musculus) IF(Homo sapiens, Mus musculus) Co-IP(Chlorocebus aethiops) IF(Chlorocebus aethiops)