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TMPRSS2 Rabbit mAb (A9126)

Publications (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:TMPRSS2 Rabbit mAb
ABclonal:Western blot - TMPRSS2 Rabbit mAb (A9126)

Western blot analysis of various lysates using TMPRSS2 Rabbit mAb (A9126) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - TMPRSS2 Rabbit mAb (A9126)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using TMPRSS2 Rabbit mAb (A9126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameTMPRSS2 Rabbit mAb
Catalog No.A9126
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1439
This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. This protein also facilitates entry of viruses into host cells by proteolytically cleaving and activating viral envelope glycoproteins. Viruses found to use this protein for cell entry include Influenza virus and the human coronaviruses HCoV-229E, MERS-CoV, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human TMPRSS2 (O15393).
SequenceMALNSGSPPAIGPYYENHGYQPENPYPAQPTVVPTVYEVHPAQYYPSPVPQYAPRVLTQASNPVVCTQPKSPSGTVCTSKTKKALCITLTLGTFLVGAAL
Gene ID
Swiss Prot
SynonymsPRSS10; TMPRSS2
Calculated MW54kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHT-29, A549, PC-3, Mouse kidney, Mouse stomach, Rat kidney
Cellular locationCell membrane, Secreted, Single-pass type II membrane protein
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - TMPRSS2 Rabbit mAb (A9126)}

Western blot - TMPRSS2 Rabbit mAb (A9126)

Western blot analysis of various lysates using TMPRSS2 Rabbit mAb (A9126) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - TMPRSS2 Rabbit mAb (A9126)}

Immunohistochemistry - TMPRSS2 Rabbit mAb (A9126)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using TMPRSS2 Rabbit mAb (A9126) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A9126? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)