제품 > 항체 > 다중클론항체(pAb)

TNF-α Rabbit pAb (A11534)

Review (1)Publications (180) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:TNF-α Rabbit pAb
ABclonal:Western blot - TNF-α Rabbit pAb (A11534)

Western blot analysis of lysates from THP-1 cells, using TNF-α Rabbit pAb (A11534) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TNF-α Rabbit pAb (A11534) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry analysis of paraffin-embedded Human liver using TNF-α Rabbit pAb (A11534) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry analysis of paraffin-embedded Human spleen using TNF-α Rabbit pAb (A11534) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A11534)

Immunofluorescence analysis of L929 cells using TNF-α Rabbit pAb (A11534) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for TNF-α Rabbit pAb(A11534)

Overview

Product nameTNF-α Rabbit pAb
Catalog No.A11534
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 134-233 of human TNF-α (NP_000585.2).
SequenceIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Gene ID
Swiss Prot
SynonymsDIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; TNF-α
Calculated MW26kDa
Observed MW18kDa/25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1 treated by PMA and LPS
Cellular locationCell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein.
Customer validation

(Mouse breast cancer)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, quails, Gallus gallus, Bos taurus, Mallotus apelta, Mus musculus)

IHC(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus)

IF(Homo sapiens, Rattus norvegicus, Mus musculus)

IHC(Mus musculus, Rattus norvegicus)

IF(Rattus norvegicus, Mus musculus, Homo sapiens, Mus musculus)

ELISA(Rattus norvegicus)

ELISA(Mus musculus, Ovis aries)

ChIP(Homo sapiens, Mus musculus)

WB(Rattus norvegicus)

WB (Rattus norvegicus)

PCR(Rattus norvegicus)

Other(Mus musculus)

IHC(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - TNF-α Rabbit pAb (A11534)}

Western blot - TNF-α Rabbit pAb (A11534)

Western blot analysis of lysates from THP-1 cells, using TNF-α Rabbit pAb (A11534) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534)}

Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TNF-α Rabbit pAb (A11534) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534)}

Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry analysis of paraffin-embedded Human liver using TNF-α Rabbit pAb (A11534) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TNF-α Rabbit pAb (A11534)}

Immunohistochemistry - TNF-α Rabbit pAb (A11534)

Immunohistochemistry analysis of paraffin-embedded Human spleen using TNF-α Rabbit pAb (A11534) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - TNF-α Rabbit pAb (A11534)}

Immunofluorescence - TNF-α Rabbit pAb (A11534)

Immunofluorescence analysis of L929 cells using TNF-α Rabbit pAb (A11534) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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ELISA 키트 (3)

재조합 단백질 (2)

제품 (7)

항체 (6)

Secondary Antibodies (26)