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TROP-2 Rabbit mAb (A20824)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TROP-2 Rabbit mAb (A20824)

Western blot analysis of various lysates using TROP-2 Rabbit mAb (A20824) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - TROP-2 Rabbit mAb (A20824)

Western blot analysis of various lysates using TROP-2 Rabbit mAb (A20824) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human esophageal tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - TROP-2 Rabbit mAb (A20824)

Confocal imaging of MCF7 cells using TROP-2 Rabbit mAb (A20824, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product nameTROP-2 Rabbit mAb
Catalog No.A20824
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51513
This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 27-274 of human TROP-2 (NP_002344.2).
SequenceHTAAQDNCTCPTNKMTVCSPDGPGGRCQCRALGSGMAVDCSTLTSKCLLLKARMSAPKNARTLVRPSEHALVDNDGLYDPDCDPEGRFKARQCNQTSVCWCVNSVGVRRTDKGDLSLRCDELVRTHHILIDLRHRPTAGAFNHSDLDAELRRLFRERYRLHPKFVAAVHYEQPTIQIELRQNTSQKAAGDVDIGDAAYYFERDIKGESLFQGRGGLDLRVRGEPLQVERTLIYYLDEIPPKFSMKRLT
Gene ID
Swiss Prot
SynonymsEGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1; TROP-2
Calculated MW36kDa
Observed MW40-65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:2000 - 1:10000
  • IF/ICC 1:100 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7 cells, A-431 cells, Mouse testis, Mouse kidney, Rat kidney
Cellular locationMembrane, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - TROP-2 Rabbit mAb (A20824)}

Western blot - TROP-2 Rabbit mAb (A20824)

Western blot analysis of various lysates using TROP-2 Rabbit mAb (A20824) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - TROP-2 Rabbit mAb (A20824)}

Western blot - TROP-2 Rabbit mAb (A20824)

Western blot analysis of various lysates using TROP-2 Rabbit mAb (A20824) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)}

Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)}

Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human esophageal tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)}

Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)}

Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)}

Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)}

Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human prostate cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)}

Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TROP-2 Rabbit mAb (A20824)}

Immunohistochemistry - TROP-2 Rabbit mAb (A20824)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using TROP-2 Rabbit mAb (A20824) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - TROP-2 Rabbit mAb (A20824)}

Immunofluorescence - TROP-2 Rabbit mAb (A20824)

Confocal imaging of MCF7 cells using TROP-2 Rabbit mAb (A20824, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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