Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of various lysates using TRPM4 Rabbit mAb (A25511) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HL-60.
Exposure time: 45s.
| Product name | TRPM4 Rabbit mAb |
|---|---|
| Catalog No. | A25511 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC65174 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1097-1214 of human TRPM4 (NP_060106.2). |
|---|---|
| Sequence | RSPQPSSPALEHFRVYLSKEAERKLLTWESVHKENFLLARARDKRESDSERLKRTSQKVDLALKQLGHIREYEQRLKVLEREVQQCSRVLGWVAEALSRSALLPPGGPPPPDLPGSKD |
| Gene ID | |
| Swiss Prot | |
| Synonyms | EKVP6; LTrpC4; PFHB1B; TRPM4B; hTRPM4 |
| Calculated MW | 134kDa |
| Observed MW | 134kDa-150KDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | MCF7 |
| Cellular location | Cell membrane, Endoplasmic reticulum, Golgi apparatus, Golgi apparatus, Multi-pass membrane protein. |
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