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TXNIP Rabbit pAb (A9342)

Reviews (2)Publications (13) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TXNIP Rabbit pAb (A9342)

Western blot analysis of lysates from Mouse thymus, using TXNIP Rabbit pAb (A9342) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - TXNIP Rabbit pAb (A9342)

Immunofluorescence analysis of U2OS cells using TXNIP Rabbit pAb (A9342) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TXNIP Rabbit pAb (A9342)

Immunofluorescence analysis of NIH/3T3 cells using TXNIP Rabbit pAb (A9342) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TXNIP Rabbit pAb (A9342)

Immunofluorescence analysis of PC-12 cells using TXNIP Rabbit pAb (A9342) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for TXNIP Rabbit pAb(A9342)
Review
ABclonal: review for TXNIP Rabbit pAb(A9342)

Overview

Product nameTXNIP Rabbit pAb
Catalog No.A9342
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a thioredoxin-binding protein that is a member of the alpha arrestin protein family. Thioredoxin is a thiol-oxidoreductase that is a major regulator of cellular redox signaling which protects cells from oxidative stress. This protein inhibits the antioxidative function of thioredoxin resulting in the accumulation of reactive oxygen species and cellular stress. This protein also functions as a regulator of cellular metabolism and of endoplasmic reticulum (ER) stress. This protein may also function as a tumor suppressor. Alternate splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-166 of human TXNIP (NP_006463.3).
SequenceMVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVIVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTSEYLRYEDTLLLEDQPTGENEMVIMRPGNKYEYKFGFELPQGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQETKKNFEVVDLVDVNTPDLMAPVSAKKEK
Gene ID
Swiss Prot
SynonymsTHIF; VDUP1; ARRDC6; HHCPA78; EST01027; TXNIP
Calculated MW44kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse thymus
Cellular locationCytoplasm
Customer validation

(Human bone marrow cancer cell, Human chronic myeloid leukemia cells)

WB(Mus musculus, Homo sapiens, Gallus gallus, Other)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

WB(Rattus norvegicus)

IF(Other)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - TXNIP Rabbit pAb (A9342)}

Western blot - TXNIP Rabbit pAb (A9342)

Western blot analysis of lysates from Mouse thymus, using TXNIP Rabbit pAb (A9342) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - TXNIP Rabbit pAb (A9342)}

Immunofluorescence - TXNIP Rabbit pAb (A9342)

Immunofluorescence analysis of U2OS cells using TXNIP Rabbit pAb (A9342) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TXNIP Rabbit pAb (A9342)}

Immunofluorescence - TXNIP Rabbit pAb (A9342)

Immunofluorescence analysis of NIH/3T3 cells using TXNIP Rabbit pAb (A9342) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TXNIP Rabbit pAb (A9342)}

Immunofluorescence - TXNIP Rabbit pAb (A9342)

Immunofluorescence analysis of PC-12 cells using TXNIP Rabbit pAb (A9342) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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ELISA 키트 (1)

항체 (1)

Secondary Antibodies (26)