Description
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands. The target strands need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37°C–75°C).
It is applicable to Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction, as well as Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification.
Workflow Chart
Storage
-80°C
Product Components
| Components | 2, 000 U | 10, 000 U |
| Taq DNA Ligase (40, 000 U/ml) | 50 μL | 250 μL |
| 10X Taq DNA Ligase Reaction Buffer | 150 μL | 650 μL |
Documents
###RM20506|||10X <i>Taq</i> DNA Ligase Reaction Buffer###RM20131)
