Reviews (8)Publications (67) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Western blot analysis of various lysates, using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of Arabidopsis thaliana (Before inflorescence), using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from C2C12 cells using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:50000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (A2357) at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Chromatin immunoprecipitation analysis of extracts of 293F cells, using TriMethyl-Histone H3-K4 Rabbit pAb antibody (A2357) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Product name | TriMethyl-Histone H3-K4 Rabbit pAb |
---|---|
Catalog No. | A2357 |
Host species | Rabbit |
Purification method | Affinity purification |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1). |
---|---|
Sequence | MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY |
Gene ID | |
Swiss Prot | |
Synonyms | H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K4 |
Calculated MW | 16kDa |
Observed MW | 17kDa/ |
Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) |
---|---|
Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
Recommended dilution |
|
Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
Key application | Western blotting Immunohistochemistry |
Positive samples | HeLa, NIH/3T3, C6, H3, Arabidopsis thaliana (Before inflorescence), C2C12 |
Cellular location | Chromosome, Nucleus. |
Customer validation | (Human cells, Plant samples, HEK293T, Mouse E16.5 neocortex, Raw264.7, Mouse Bone marrow stromal cells, Mouse primary calvarial cells) WB(Rattus norvegicus, Mus musculus, Homo sapiens, Drosophila, Eurosta solidaginis, Danio rerio, Oryza sativa, Saccharomyces cerevisiae, Other) IF(Homo sapiens, Oryza sativa, Mus musculus) IP(Homo sapiens, Mus musculus) IHC(Mus musculus, Bombyx mori Linnaeus) CUT&Tag(Homo sapiens, Oryza sativa, Other, Mus musculus) ChIP(Homo sapiens, Oryza sativa) ChIP-Seq(Homo sapiens, Zea mays, Other, Arabidopsis thaliana, Oryza sativa, Triticum aestivum) ChIP(Homo sapiens, Mus musculus, Oryza sativa, Other, Arabidopsis thaliana, Rattus norvegicus, Zea mays) CUT&Tag(Oryza sativa) IF(Oryza sativa) IHC(Rattus norvegicus) IF(Rattus norvegicus) WB(Mus musculus) WB (Mus musculus) |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr
Publishing research using A2357? Please let us know so that we can cite the reference in this datasheet.
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K9 Rabbit pAb
A2360
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K14 Rabbit pAb
A7254
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K18 Rabbit pAb
A7257
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K56 Rabbit pAb
A7256
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb
A2421
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K27 Rabbit pAb
A2361
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S10 Rabbit pAb
AP0840
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K36 Rabbit pAb
A2364
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K79 Rabbit pAb
A2369
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R8 Rabbit pAb
A2374
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb
A3155
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K36 Rabbit mAb
A20379
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R2 Rabbit pAb
A2373
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K4 Rabbit pAb
A16078
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K27 Rabbit mAb
A2771
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S10 Rabbit mAb
AP0002
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb
A17917
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Lactic acid-Histone H3-K18 Rabbit pAb
A18807
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K4 Rabbit mAb
A22078
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R26 Rabbit pAb
A3163
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R2 Rabbit pAb
A3154
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R26 Rabbit pAb
A2375
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K4 Rabbit pAb
A17019
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K36 Rabbit pAb
A16077
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Lactic acid-Histone H3-K14 Rabbit pAb
A18808
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T45 Rabbit pAb
AP0898
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Lactic acid-Histone H3-K27 Rabbit pAb
A18825
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K36 Rabbit pAb
A20566
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K79 Rabbit pAb
A20822
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K36 Rabbit mAb
A22087
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K4 Rabbit mAb
A22225
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K36 Rabbit mAb
A22863
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K27 Rabbit mAb
A22396
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K9 Rabbit mAb
A26196
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K23 Rabbit mAb
A2770
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R8 Rabbit pAb
A3156
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T11 Rabbit pAb
AP0093
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K56 Rabbit pAb
A7262
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K64 Rabbit pAb
A7259
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K36 Rabbit mAb
A22221
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K36 Rabbit mAb
A23944
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K14 Rabbit pAb
A5278
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K14 Rabbit pAb
A5279
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K14 Rabbit pAb
A5277
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R8 Rabbit pAb
A3157
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R26 Rabbit pAb
A3153
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R17 Rabbit pAb
A3151
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R17 Rabbit pAb
A3152
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb
A20732
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K27 Rabbit mAb
A22006
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R17 Rabbit mAb
A22291
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb
A21299
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S28 Rabbit pAb
AP0839
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T3 Rabbit pAb
AP0846
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S10/T11 Rabbit pAb
AP0896
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T32 Rabbit pAb
AP0897
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T6 Rabbit pAb
AP0899
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K23 Rabbit pAb
A18154
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R2 Rabbit mAb
A19645
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb
A17899
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3.3-T3 Rabbit mAb
AP1152
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K36 Rabbit pAb
A20185
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K27 Rabbit pAb
A20184
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R8 Rabbit pAb
A20820
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K79 Rabbit pAb
A20821
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Lactic acid-Histone H3-K18 Rabbit mAb
A21214
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R8 Rabbit mAb
A21207
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K79 Rabbit mAb
A21223
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb
A22144
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K9 Rabbit mAb
A22079
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K27 Rabbit mAb
A22170
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K4 Rabbit mAb
A22224
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 Rabbit pAb
A21295
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K4 Rabbit mAb
A24341
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K18 Rabbit mAb
A22566
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S28 Rabbit mAb
AP1431
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)