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TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

Reviews (8)Publications (67) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:TriMethyl-Histone H3-K4 Rabbit pAb
ABclonal:Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

Western blot analysis of various lysates, using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

Western blot analysis of Arabidopsis thaliana (Before inflorescence), using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

Western blot analysis of lysates from C2C12 cells using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:50000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Dot Blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (A2357) at 1:1000 dilution.

ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

Chromatin immunoprecipitation analysis of extracts of 293F cells, using TriMethyl-Histone H3-K4 Rabbit pAb antibody (A2357) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for TriMethyl-Histone H3-K4 Rabbit pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 Rabbit pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 Rabbit pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 Rabbit pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 Rabbit pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 Rabbit pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 Rabbit pAb(A2357)
Review
ABclonal: review for TriMethyl-Histone H3-K4 Rabbit pAb(A2357)

Overview

Product nameTriMethyl-Histone H3-K4 Rabbit pAb
Catalog No.A2357
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).
SequenceMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Gene ID
Swiss Prot
SynonymsH3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K4
Calculated MW16kDa
Observed MW17kDa/
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • DB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
  • ChIP-seq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, NIH/3T3, C6, H3, Arabidopsis thaliana (Before inflorescence), C2C12
Cellular locationChromosome, Nucleus.
Customer validation

(Human cells, Plant samples, HEK293T, Mouse E16.5 neocortex, Raw264.7, Mouse Bone marrow stromal cells, Mouse primary calvarial cells)

WB(Rattus norvegicus, Mus musculus, Homo sapiens, Drosophila, Eurosta solidaginis, Danio rerio, Oryza sativa, Saccharomyces cerevisiae, Other)

IF(Homo sapiens, Oryza sativa, Mus musculus)

IP(Homo sapiens, Mus musculus)

IHC(Mus musculus, Bombyx mori Linnaeus)

CUT&Tag(Homo sapiens, Oryza sativa, Other, Mus musculus)

ChIP(Homo sapiens, Oryza sativa)

ChIP-Seq(Homo sapiens, Zea mays, Other, Arabidopsis thaliana, Oryza sativa, Triticum aestivum)

ChIP(Homo sapiens, Mus musculus, Oryza sativa, Other, Arabidopsis thaliana, Rattus norvegicus, Zea mays)

CUT&Tag(Oryza sativa)

IF(Oryza sativa)

IHC(Rattus norvegicus)

IF(Rattus norvegicus)

WB(Mus musculus)

WB (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)}

    Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

    Western blot analysis of various lysates, using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:400 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)}

    Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

    Western blot analysis of Arabidopsis thaliana (Before inflorescence), using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:400 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)}

    Western blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

    Western blot analysis of lysates from C2C12 cells using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at 1:50000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Dot Blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)}

    Dot Blot - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

    Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (A2357) at 1:1000 dilution.
    ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)}

    Immunohistochemistry - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)}

    Immunohistochemistry - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TriMethyl-Histone H3-K4 Rabbit pAb (A2357) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)}

    Chromatin Immunoprecipitation - TriMethyl-Histone H3-K4 Rabbit pAb (A2357)

    Chromatin immunoprecipitation analysis of extracts of 293F cells, using TriMethyl-Histone H3-K4 Rabbit pAb antibody (A2357) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    항체 (105)

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