Reviews (6)Publications (49) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Western blot analysis of various lysates using TriMethyl-Histone H3-K9 Rabbit pAb (A2360) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody (A2360).
Immunohistochemistry analysis of paraffin-embedded Rat testis using TriMethyl-Histone H3-K9 Rabbit pAb (A2360) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using TriMethyl-Histone H3-K9 Rabbit pAb (A2360) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K9 Rabbit pAb (A2360) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF7 cells using TriMethyl-Histone H3-K9 Rabbit pAb (A2360) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K9 Rabbit pAb (A2360) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K9 Rabbit pAb (A2360) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K9 antibody (A2360) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| Product name | TriMethyl-Histone H3-K9 Rabbit pAb |
|---|---|
| Catalog No. | A2360 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic trimethylated peptide around K9 of human histone H3 (NP_003520.1). |
|---|---|
| Sequence | ARKST |
| Gene ID | |
| Swiss Prot | |
| Synonyms | H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K9 |
| Calculated MW | 16kDa |
| Observed MW | 17kDa |
| Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H3 protein |
| Cellular location | Chromosome, Nucleus. |
| Customer validation | (mouse round spermatid cells, 293, Goat testis, Goat sperm, HEK293T) WB(, Mus musculus, Homo sapiens, Drosophila, Mus musculus, Rattus norvegicus) IF(Mus musculus, Homo sapiens, Oryctolagus cuniculus, Rattus norvegicus) IHC(Homo sapiens) IF(Homo sapiens, Mus musculus, Mus musculus, Rattus norvegicus) ChIP(Mus musculus, Sus scrofa, Homo sapiens) IHC(Mus musculus, Rattus norvegicus) IP(Mus musculus) Other(Homo sapiens) ChIP(Rattus norvegicus) PCR(Homo sapiens,Mus musculus) |
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_WB_01.jpg?t=1721357921 "ABclonal:Western blot - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
_DB_01.jpg?t=1721357921 "ABclonal:Dot Blot - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
(Me)(AX)_IHC_01.jpg?t=1721357921 "ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
(Me)(AX)_IHC_02.jpg?t=1721357921 "ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
_IF_01.jpg?t=1721357921 "ABclonal:Immunofluorescence - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
_IF_02.jpg?t=1721357921 "ABclonal:Immunofluorescence - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
_IF_03.jpg?t=1721357921 "ABclonal:Immunofluorescence - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
_IF_04.jpg?t=1721357921 "ABclonal:Immunofluorescence - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
_ChIP_01.jpg?t=1721357921 "ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K9 Rabbit pAb (A2360)")
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