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α-Tubulin Mouse mAb (AC012)

Review (1)Publications (91) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:α-Tubulin Mouse mAb
ABclonal:Western blot - α-Tubulin Mouse mAb (AC012)

Western blot analysis of various lysates using α-Tubulin Mouse mAb (AC012) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - α-Tubulin Mouse mAb (AC012)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using α-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - α-Tubulin Mouse mAb (AC012)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using α-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - α-Tubulin Mouse mAb (AC012)

Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:200)(Green). The cells were counterstained with USO1 Rabbit mAb (A20950, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Review
ABclonal: review for α-Tubulin Mouse mAb(AC012)

Overview

Product nameα-Tubulin Mouse mAb
Catalog No.AC012
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0479
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant protein of human α-Tubulin
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsALS22; TUBA1; H2-ALPHA; α-Tubulin
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:100 - 1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesU-87MG, 293T, HeLa, Jurkat, Mouse spleen, Mouse thymus, Mouse brain, Rat brain, Rat kidney
Cellular locationCytoplasm, cytoskeleton.
Customer validation

(HeLa treated by Starvatio and IGF)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Tegillarca granosa, Danio rerio, Artemisia scoparia, Sus domesticus, Capra hircus, Mus musculus, )

IP(Homo sapiens)

IF(Mus musculus, Homo sapiens)

IP(Homo sapiens)

IHC(Homo sapiens, Mus musculus,)

Co-IP(Homo sapiens)

WB(Homo sapiens)

ChIP(Homo sapiens)

Co-IP(Homo sapiens)

FC(Homo sapiens)

IF(Homo sapiens)

RT-qPCR(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - α-Tubulin Mouse mAb (AC012)}

Western blot - α-Tubulin Mouse mAb (AC012)

Western blot analysis of various lysates using α-Tubulin Mouse mAb (AC012) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - α-Tubulin Mouse mAb (AC012)}

Immunohistochemistry - α-Tubulin Mouse mAb (AC012)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using α-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - α-Tubulin Mouse mAb (AC012)}

Immunohistochemistry - α-Tubulin Mouse mAb (AC012)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using α-Tubulin Mouse mAb (AC012) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - α-Tubulin Mouse mAb (AC012)}

Immunofluorescence - α-Tubulin Mouse mAb (AC012)

Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:200)(Green). The cells were counterstained with USO1 Rabbit mAb (A20950, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

* For research use only. Not for therapeutic or diagnostic purposes.

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