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β-Tubulin Mouse mAb (AC021)

Publications (72) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii

ABclonal:β-Tubulin Mouse mAb
ABclonal:Western blot - β-Tubulin Mouse mAb (AC021)

Western blot analysis of various lysates using β-Tubulin Mouse mAb (AC021) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry analysis of paraffin-embedded Rat stomach using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameβ-Tubulin Mouse mAb
Catalog No.AC021
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0498
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
ImmunogenRecombinant protein of human β-Tubulin
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsM40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; β-Tubulin
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesSH-SY5Y, MCF-7, HepG2, HeLa, Mouse brain, Mouse lung, Rat brain
Cellular locationCytoplasm, cytoskeleton.
Customer validation

WB(, Homo sapiens, Mus musculus, Rattus norvegicus, Arabidopsis thaliana, Mesocricetus auratus, Danio rerio, Mus musculus, Rattus norvegicus)

IF(Mus musculus, Homo sapiens)

IF(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

IP(Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - β-Tubulin Mouse mAb (AC021)}

Western blot - β-Tubulin Mouse mAb (AC021)

Western blot analysis of various lysates using β-Tubulin Mouse mAb (AC021) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)}

Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Tubulin Mouse mAb (AC021)}

Immunohistochemistry - β-Tubulin Mouse mAb (AC021)

Immunohistochemistry analysis of paraffin-embedded Rat stomach using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)}

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of C6 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)}

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Mouse mAb (AC021)}

Immunofluorescence - β-Tubulin Mouse mAb (AC021)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Mouse mAb (AC021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AC021? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

Secondary Antibodies (22)