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β-Tubulin Rabbit pAb (AC008)

Publications (147) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:β-Tubulin Rabbit pAb
ABclonal:Western blot - β-Tubulin Rabbit pAb (AC008)

Western blot analysis of various lysates, using β-Tubulin Rabbit pAb (AC008) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry analysis of paraffin-embedded Human liver using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of C6 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameβ-Tubulin Rabbit pAb
Catalog No.AC008
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human β-Tubulin (NP_006077.2).
SequenceSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF
Gene ID
Swiss Prot
SynonymsM40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56; β-Tubulin
Calculated MW50kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, SH-SY5Y, Mouse brain, Rat brain
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Mus musculus, Crassostrea gigas, Homo sapiens, Rattus norvegicus, Oncorhynchus mykiss, Brachyura, Capra hircus, Eriocheir sinensis H.Milne-Edwards, Gallus gallus, Sus scrofa, Yellow catfish, Mus musculus, Larimichthys crocea, blunt snout bream, Other, Arabidopsis thaliana, Schizothorax prenanti, Oryctolagus cuniculus)

IHC(Rattus norvegicus, Mus musculus)

IF(Mus musculus, Rattus norvegicus, Homo sapiens)

IF(Homo sapiens, Mus musculus)

IP(Homo sapiens)

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - β-Tubulin Rabbit pAb (AC008)}

Western blot - β-Tubulin Rabbit pAb (AC008)

Western blot analysis of various lysates, using β-Tubulin Rabbit pAb (AC008) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)}

Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry analysis of paraffin-embedded Human liver using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)}

Immunohistochemistry - β-Tubulin Rabbit pAb (AC008)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using β-Tubulin Rabbit pAb (AC008) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)}

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of C6 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)}

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of NIH-3T3 cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-Tubulin Rabbit pAb (AC008)}

Immunofluorescence - β-Tubulin Rabbit pAb (AC008)

Immunofluorescence analysis of U-2 OS cells using β-Tubulin Rabbit pAb (AC008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AC008? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

β-Tubulin Mouse mAb
AC021

ELISAWBIHC-PIF/ICCIP

Reactivity:Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii

β-Tubulin Mouse mAb

β-Tubulin Rabbit mAb
A12289

WBIHC-PIF/ICCIPELISA

Reactivity:Human, Mouse, Rat

β-Tubulin Rabbit mAb

β-Tubulin Rabbit pAb
AC015

WBIHC-PIF/ICC

Reactivity:Human, Mouse, Rat

β-Tubulin Rabbit pAb

HRP-conjugated β-Tubulin Mouse mAb
AC030

WBELISA

Reactivity:Human, Mouse, Rat

HRP-conjugated β-Tubulin Mouse mAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)