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Tyrosine Hydroxylase Rabbit pAb (A0028)

Publications (12) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Tyrosine Hydroxylase Rabbit pAb (A0028)

Western blot analysis of lysates from Jurkat cells using Tyrosine Hydroxylase Rabbit pAb (A0028) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Tyrosine Hydroxylase Rabbit pAb (A0028)

Immunofluorescence analysis of Neuro-2a cells using Tyrosine Hydroxylase Rabbit pAb (A0028) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Tyrosine Hydroxylase Rabbit pAb (A0028)

Immunofluorescence analysis of SH-SY5Y cells using Tyrosine Hydroxylase Rabbit pAb (A0028) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTyrosine Hydroxylase Rabbit pAb
Catalog No.A0028
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
ImmunogenA synTyrosine Hydroxylase etic peptide corresponding to a sequence wiTyrosine Hydroxylase in amino acids 411-528 of human Tyrosine Hydroxylase (NP_954986.2).
SequenceKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG
Gene ID
Swiss Prot
SynonymsTYH; DYT14; DYT5b; Tyrosine Hydroxylase
Calculated MW59kDa
Observed MW59kDa/58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain, Jurkat
Cellular locationaxon, cytoplasm, cytoplasmic side of plasma membrane, cytoplasmic vesicle, cytosol, dendrite, mitochondrion, neuron projection, nucleus, perikaryon, perinuclear region of cytoplasm, smooth endoplasmic reticulum, synaptic vesicle, terminal bouton.
Customer validation

IF(Mus musculus, Homo sapiens)

WB(Rattus norvegicus, Mus musculus)

IHC(Rattus norvegicus, Mus musculus)

IHC(Mus musculus)

IF(Rattus norvegicus)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Tyrosine Hydroxylase Rabbit pAb (A0028)}

Western blot - Tyrosine Hydroxylase Rabbit pAb (A0028)

Western blot analysis of lysates from Jurkat cells using Tyrosine Hydroxylase Rabbit pAb (A0028) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Tyrosine Hydroxylase Rabbit pAb (A0028)}

Immunofluorescence - Tyrosine Hydroxylase Rabbit pAb (A0028)

Immunofluorescence analysis of Neuro-2a cells using Tyrosine Hydroxylase Rabbit pAb (A0028) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Tyrosine Hydroxylase Rabbit pAb (A0028)}

Immunofluorescence - Tyrosine Hydroxylase Rabbit pAb (A0028)

Immunofluorescence analysis of SH-SY5Y cells using Tyrosine Hydroxylase Rabbit pAb (A0028) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (4)

Secondary Antibodies (26)