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UGT1A1 Rabbit pAb (A6186)

Reviews (2)Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UGT1A1 Rabbit pAb (A6186)

Western blot analysis of lysates from Rat liver, using UGT1A1 Rabbit pAb (A6186) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - UGT1A1 Rabbit pAb (A6186)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A6186)

Immunofluorescence analysis of C6 cells using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A6186)

Immunofluorescence analysis of HepG2 cells using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A6186)

Immunofluorescence analysis of paraffin-embedded human liver using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A6186)

Immunofluorescence analysis of paraffin-embedded rat liver using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for UGT1A1 Rabbit pAb(A6186)
Review
ABclonal: review for UGT1A1 Rabbit pAb(A6186)

Overview

Product nameUGT1A1 Rabbit pAb
Catalog No.A6186
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UGT1A1 (NP_000454.1).
SequenceMAVESQGGRPLVLGLLLCVLGPVVSHAGKILLIPVDGSHWLSMLGAIQQLQQRGHEIVVLAPDASLYIRDGAFYTLKTYPVPFQREDVKESFVSLGHNVFENDSFLQRVIKTYKKIKKDSAMLLSGCSHLLHNKELMASLAESSFDVMLTDPFLPCSPIVAQYLSLPTVFFLHALPCSLEFEATQCPNPFSYVPRPLSSH
Gene ID
Swiss Prot
SynonymsGNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1; UGT1A1
Calculated MW60kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat liver
Cellular locationEndoplasmic reticulum, Endoplasmic reticulum membrane, Microsome, Single-pass membrane protein.
Customer validation

(Mouse liver, unknow)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - UGT1A1 Rabbit pAb (A6186)}

Western blot - UGT1A1 Rabbit pAb (A6186)

Western blot analysis of lysates from Rat liver, using UGT1A1 Rabbit pAb (A6186) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - UGT1A1 Rabbit pAb (A6186)}

Immunohistochemistry - UGT1A1 Rabbit pAb (A6186)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A6186)}

Immunofluorescence - UGT1A1 Rabbit pAb (A6186)

Immunofluorescence analysis of C6 cells using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A6186)}

Immunofluorescence - UGT1A1 Rabbit pAb (A6186)

Immunofluorescence analysis of HepG2 cells using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A6186)}

Immunofluorescence - UGT1A1 Rabbit pAb (A6186)

Immunofluorescence analysis of paraffin-embedded human liver using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - UGT1A1 Rabbit pAb (A6186)}

Immunofluorescence - UGT1A1 Rabbit pAb (A6186)

Immunofluorescence analysis of paraffin-embedded rat liver using UGT1A1 Rabbit pAb (A6186) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)